Einzeltreffer — DigiBib

We compared the contribution of IL-17A and IL-17F in co-culture systems mimicking cell interactions as found in inflamed synovium and skin. Synoviocytes or skin fibroblasts were co-cultured with activated PBMC, with IL-17A, IL-17 A/F, IL-17F, IL-23, anti-IL-17A, anti-IL-17A/F or anti-IL-17F antibodies. IL-17A, IL-17F, IL-6 and IL-10 production was measured at 48 h. mRNA expression of receptor subunits for IL-23, IL-12 and IL-17 was assessed at 24 h. Both cell activation and interactions were needed for a high IL-17A secretion while IL-17F was stimulated by PHA activation alone and further increased in co-cultures. IL-17F levels were higher than IL-17A in both co-cultures (p < 0.05). IL-17F addition decreased IL-17A secretion (p < 0.05) but IL-17A addition had no effect on IL-17F secretion. Interestingly, IL-17A and IL-17F upregulated IL-17RA and IL-17RC mRNA expression in PBMC/skin fibroblast co-cultures (p < 0.05) while only IL-17F exerted this effect in synoviocytes (p < 0.05). Monocyte exclusion in both co-cultures increased IL-17A and IL-17F (twofold, p < 0.05) while decreasing IL-10 and IL-6 secretion (twofold, p < 0.05). IL-17A and F had differential effects on their receptor expression with a higher sensitivity for skin fibroblasts highlighting the differential contribution of IL-17A and F in joint vs. skin diseases.

Titel:	Differential effects of interleukin-17A and 17F on cell interactions between immune cells and stromal cells from synovium or skin.
Autor/in / Beteiligte Person:	Tout, I ; Noack, M ; Miossec, P
Link:	Full Text Finder (Volltext)
Zeitschrift:	Scientific reports, Jg. 13 (2023-11-06), Heft 1, S. 19223
Veröffentlichung:	London : Nature Publishing Group, copyright 2011-, 2023
Medientyp:	academicJournal
ISSN:	2045-2322 (electronic)
DOI:	10.1038/s41598-023-45653-8
Schlagwort:	Leukocytes, Mononuclear metabolism Interleukin-6 metabolism Cell Communication Synovial Membrane metabolism Stromal Cells metabolism RNA, Messenger metabolism Interleukin-23 metabolism Interleukin-17 metabolism Interleukin-10 metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Sci Rep] 2023 Nov 06; Vol. 13 (1), pp. 19223. <i>Date of Electronic Publication: </i>2023 Nov 06. MeSH Terms: Interleukin-17* / metabolism ; Interleukin-10* / metabolism ; Leukocytes, Mononuclear / metabolism ; Interleukin-6 / metabolism ; Cell Communication ; Synovial Membrane / metabolism ; Stromal Cells / metabolism ; RNA, Messenger / metabolism ; Interleukin-23 / metabolism References: J Allergy Clin Immunol. 2017 Mar;139(3):923-932.e8. (PMID: 27502297) ; J Mol Med (Berl). 2018 Aug;96(8):819-829. (PMID: 29959474) ; N Engl J Med. 2009 Aug 27;361(9):888-98. (PMID: 19710487) ; Ann Rheum Dis. 2015 Aug;74(8):1629-31. (PMID: 25902792) ; Ann Rheum Dis. 2013 Apr;72 Suppl 2:ii116-23. (PMID: 23253932) ; J Immunol. 2008 Jun 1;180(11):7423-30. (PMID: 18490742) ; Front Med (Lausanne). 2019 Jan 14;5:364. (PMID: 30693283) ; Immunity. 2020 Mar 17;52(3):499-512.e5. (PMID: 32187518) ; Eur J Immunol. 2020 Apr;50(4):568-580. (PMID: 31850514) ; J Immunol. 2006 Jul 1;177(1):36-9. (PMID: 16785495) ; Nat Immunol. 2007 Sep;8(9):942-9. (PMID: 17676045) ; Clin Exp Immunol. 2016 Oct;186(1):64-74. (PMID: 27328392) ; Sci Transl Med. 2010 Oct 6;2(52):52ra72. (PMID: 20926833) ; Cell Rep. 2022 Oct 18;41(3):111489. (PMID: 36260993) ; Front Immunol. 2021 Feb 22;12:637829. (PMID: 33692806) ; J Exp Med. 1993 Feb 1;177(2):523-7. (PMID: 8426121) ; Front Immunol. 2016 Nov 17;7:509. (PMID: 27909436) ; Biomed Res Int. 2014;2014:698313. (PMID: 24524085) ; Autoimmun Rev. 2022 Jun;21(6):103098. (PMID: 35417796) ; Rheumatology (Oxford). 2016 Dec;55(suppl 2):ii43-ii55. (PMID: 27856660) ; J Invest Dermatol. 2014 Apr;134(4):984-991. (PMID: 24352038) ; Ann Rheum Dis. 2019 Aug;78(8):1033-1040. (PMID: 31177099) ; Front Immunol. 2020 Aug 21;11:1894. (PMID: 32973785) ; Protein Cell. 2011 Jan;2(1):26-40. (PMID: 21337007) ; Immunol Lett. 2010 Jan 4;127(2):150-6. (PMID: 19895848) ; J Rheumatol. 2015 Jun;42(6):912-9. (PMID: 25877498) ; Sci Rep. 2017 Aug 21;7(1):8906. (PMID: 28827714) ; Front Immunol. 2021 Jun 17;12:655477. (PMID: 34220809) ; Nat Rev Rheumatol. 2021 Sep;17(9):550-564. (PMID: 34345021) ; J Immunol. 2007 Oct 15;179(8):5462-73. (PMID: 17911633) ; Front Immunol. 2020 Nov 20;11:585134. (PMID: 33329560) ; Nat Rev Rheumatol. 2019 Aug;15(8):491-501. (PMID: 31227819) ; Arthritis Res Ther. 2014 Aug 22;16(4):426. (PMID: 25146432) ; J Immunol Methods. 2010 Oct 31;362(1-2):70-81. (PMID: 20833179) ; Ann Rheum Dis. 2011 Feb;70(2):341-8. (PMID: 21109515) ; J Clin Invest. 2013 Jan;123(1):247-60. (PMID: 23221338) ; Arthritis Res Ther. 2016 Jun 23;18:148. (PMID: 27338729) ; Cell Mol Immunol. 2021 Apr;18(4):860-865. (PMID: 33692481) ; J Immunol. 2009 Mar 1;182(5):3112-20. (PMID: 19234208) ; N Engl J Med. 2015 Oct;373(14):1318-28. (PMID: 26422722) ; Arthritis Res. 2002;4(1):64-70. (PMID: 11879539) ; J Immunol. 2007 Apr 1;178(7):4466-72. (PMID: 17372004) ; Annu Rev Med. 2016;67:337-53. (PMID: 26565676) ; J Rheumatol. 2020 Nov 1;47(11):1606-1613. (PMID: 31941804) ; J Exp Med. 2007 May 14;204(5):995-1001. (PMID: 17470641) ; Eur Cytokine Netw. 2014 Oct-Dec;25(4):63-8. (PMID: 25679113) ; Nat Rev Immunol. 2023 Jan;23(1):38-54. (PMID: 35790881) ; Arthritis Rheum. 2010 Sep;62(9):2569-81. (PMID: 20872595) Substance Nomenclature: 0 (Interleukin-17) ; 130068-27-8 (Interleukin-10) ; 0 (Interleukin-6) ; 0 (RNA, Messenger) ; 0 (Interleukin-23) Entry Date(s): Date Created: 20231106 Date Completed: 20231108 Latest Revision: 20231111 Update Code: 20231215 PubMed Central ID: PMC10628108

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Differential effects of interleukin-17A and 17F on cell interactions between immune cells and stromal cells from synovium or skin.