Differential effects of interleukin-17A and 17F on cell interactions between immune cells and stromal cells from synovium or skin.
In: Scientific reports, Jg. 13 (2023-11-06), Heft 1, S. 19223
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Zugriff:
We compared the contribution of IL-17A and IL-17F in co-culture systems mimicking cell interactions as found in inflamed synovium and skin. Synoviocytes or skin fibroblasts were co-cultured with activated PBMC, with IL-17A, IL-17 A/F, IL-17F, IL-23, anti-IL-17A, anti-IL-17A/F or anti-IL-17F antibodies. IL-17A, IL-17F, IL-6 and IL-10 production was measured at 48 h. mRNA expression of receptor subunits for IL-23, IL-12 and IL-17 was assessed at 24 h. Both cell activation and interactions were needed for a high IL-17A secretion while IL-17F was stimulated by PHA activation alone and further increased in co-cultures. IL-17F levels were higher than IL-17A in both co-cultures (p < 0.05). IL-17F addition decreased IL-17A secretion (p < 0.05) but IL-17A addition had no effect on IL-17F secretion. Interestingly, IL-17A and IL-17F upregulated IL-17RA and IL-17RC mRNA expression in PBMC/skin fibroblast co-cultures (p < 0.05) while only IL-17F exerted this effect in synoviocytes (p < 0.05). Monocyte exclusion in both co-cultures increased IL-17A and IL-17F (twofold, p < 0.05) while decreasing IL-10 and IL-6 secretion (twofold, p < 0.05). IL-17A and F had differential effects on their receptor expression with a higher sensitivity for skin fibroblasts highlighting the differential contribution of IL-17A and F in joint vs. skin diseases.
(© 2023. The Author(s).)
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Differential effects of interleukin-17A and 17F on cell interactions between immune cells and stromal cells from synovium or skin.
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Autor/in / Beteiligte Person: | Tout, I ; Noack, M ; Miossec, P |
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Zeitschrift: | Scientific reports, Jg. 13 (2023-11-06), Heft 1, S. 19223 |
Veröffentlichung: | London : Nature Publishing Group, copyright 2011-, 2023 |
Medientyp: | academicJournal |
ISSN: | 2045-2322 (electronic) |
DOI: | 10.1038/s41598-023-45653-8 |
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