Engineered fluorogenic HaloTag ligands for turn-on labelling in live cells.
In: Chemical communications (Cambridge, England), Jg. 60 (2023-12-21), Heft 2, S. 200-203
academicJournal
Zugriff:
Recent years have seen dramatic improvements in the design of organic fluorophores based on limiting non-radiative decay pathways. We sought to extend this understanding to benzothiadiazoles that have been used as turn-on fluorescent substrates for the self-labeling protein HaloTag. When conjugated to HaloTag, the benzothiadiazoles reside in a narrow tunnel that precludes twisted internal charge transfer, which allowed us to explore steric and electronic effects on other non-radiative decay pathways. By minimizing both non-radiative decay and nonspecific interactions with cellular components, we produced improved turn-on dyes with 136-fold increase in fluorescence over background in cells.
Titel: |
Engineered fluorogenic HaloTag ligands for turn-on labelling in live cells.
|
---|---|
Autor/in / Beteiligte Person: | Lampkin, BJ ; Kritzer, JA |
Zeitschrift: | Chemical communications (Cambridge, England), Jg. 60 (2023-12-21), Heft 2, S. 200-203 |
Veröffentlichung: | Cambridge : Royal Society of Chemistry, 2023 |
Medientyp: | academicJournal |
ISSN: | 1364-548X (electronic) |
DOI: | 10.1039/d3cc05536a |
Schlagwort: |
|
Sonstiges: |
|