Comparison of the penetration depth in mouse brain in vivo through 3PF imaging using AIE nanoparticle labeling and THG imaging within the 1700 nm window.
In: Nanoscale advances, Jg. 6 (2023-12-06), Heft 2, S. 511-515
Online
academicJournal
Zugriff:
3-Photon microscopy (3PM) excited at the 1700 nm window features a smaller tissue attenuation and hence a larger penetration depth in brain imaging compared with other excitation wavelengths in vivo . While the comparison of the penetration depth quantified by effective attenuation length l e with other excitation wavelengths have been extensively investigated, comparison within the 1700 nm window has never been demonstrated. This is mainly due to the lack of a proper excitation laser source and characterization of the in vivo emission properties of fluorescent labels within this window. Herein, we demonstrate detailed measurements and comparison of l e through the 3-photon imaging of the mouse brain in vivo , at different excitation wavelengths (1600 nm, 1700 nm, and 1800 nm). 3PF imaging and in vivo spectrum measurements were performed using AIE nanoparticle labeling. Our results show that l e derived from both 3PF imaging and THG imaging is the largest at 1700 nm, indicating that it enables the deepest penetration in brain imaging in vivo .
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Titel: |
Comparison of the penetration depth in mouse brain in vivo through 3PF imaging using AIE nanoparticle labeling and THG imaging within the 1700 nm window.
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Autor/in / Beteiligte Person: | Zhang, Y ; Zhong, J ; Cheng, H ; Huang, J ; Li, Z ; Zhang, C ; Gao, Z ; Xu, Z ; Xu, G ; Qiu, P ; Wang, K |
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Zeitschrift: | Nanoscale advances, Jg. 6 (2023-12-06), Heft 2, S. 511-515 |
Veröffentlichung: | Cambridge : Royal Society of Chemistry, [2019]-, 2023 |
Medientyp: | academicJournal |
ISSN: | 2516-0230 (electronic) |
DOI: | 10.1039/d3na00871a |
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