Einzeltreffer — DigiBib

While automated modules for F-18 and C-11 radiosyntheses are standardized with features such as multiple reactors, vacuum connection and semi-preparative HPLC, labeling and processing of compounds with radiometals such as Zr-89, Lu-177 and Ac-225 often do not require complex manipulations and are frequently performed manually by a radiochemist. These procedures typically involve transferring solutions to and from vials using pipettes followed by heating of the reaction mixture, and do not require all the features found in most commercial automated synthesis units marketed as F-18 or C-11 modules. Here we present an efficient automated method for performing radiosyntheses involving radiometals by adapting a commercially available robotic pipettor originally developed for high-throughput processing of biological samples. While a robotic pipettor is less costly than a radiosynthesis module, it holds many similar advantages over manual radiosynthesis such as minimization of operator error, lower operator exposure rates, and abbreviated synthesis times, among others. To demonstrate the feasibility of using the OpenTrons OT-2 robotic pipettor to perform automated radiosyntheses, we radiolabeled and formulated 177 Lu-PSMA-617 and 225 Ac-PSMA-617 on the system. The OT-2 was then used to help streamline the quality control process for both products, further minimizing manual handling by and exposure to the radiochemist.

Titel:	Automated radiolabeling and handling of <superscript>177</superscript> Lu- and <superscript>225</superscript> Ac-PSMA-617 using a robotic pipettor.
Autor/in / Beteiligte Person:	Ahn, SH ; Belanger, AP
Link:	Full Text Finder (Volltext)
Zeitschrift:	Journal of labelled compounds & radiopharmaceuticals, Jg. 67 (2024-03-01), Heft 3, S. 111-115
Veröffentlichung:	London, New York, Wiley., 2024
Medientyp:	academicJournal
ISSN:	1099-1344 (electronic)
DOI:	10.1002/jlcr.4085
Schlagwort:	Actinium Zirconium Radioisotopes Robotic Surgical Procedures Dipeptides Heterocyclic Compounds, 1-Ring Prostate-Specific Antigen
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Labelled Comp Radiopharm] 2024 Mar; Vol. 67 (3), pp. 111-115. <i>Date of Electronic Publication: </i>2024 Jan 31. MeSH Terms: Radioisotopes* ; Robotic Surgical Procedures* ; Dipeptides* ; Heterocyclic Compounds, 1-Ring* ; Prostate-Specific Antigen* ; Actinium ; Zirconium References: Alfteimi A, Lützen U, Helm A, et al. Automated synthesis of [68Ga]Ga-FAPI-46 without pre-purification of the generator eluate on three common synthesis modules and two generator types. EJNMMI Radiopharm Chem. 2022;7(1):20. doi:10.1186/s41181-022-00172-1. ; Poot AJ, Adamzek KWA, Windhorst AD, et al. Fully automated 89Zr labeling and purification of antibodies. J Nucl Med. 2019;60(5):691-695. doi:10.2967/jnumed.118.217158. ; Wichmann CW, Poniger S, Guo N, et al. Automated radiosynthesis of [89Zr]Zr-DFOSq-Durvalumab for imaging of PD-L1 expressing tumours in vivo. Nucl Med Biol. 2023;120-121:108351. doi:10.1016/j.nucmedbio.2023.108351. ; Fuscaldi LL, Sobral DV, Durante ACR, et al. Standardization of the [68Ga]Ga-PSMA-11 radiolabeling protocol in an automatic synthesis module: assessments for PET imaging of prostate cancer. Pharmaceuticals. 2021;14(5):385. doi:10.3390/ph14050385. ; Liu T, Redalen KR, Karlsen M. Development of an automated production process of [64Cu][Cu (ATSM)] for positron emission tomography imaging and theranostic applications. J Label Compd Radiopharm. 2022;65(7):191-202. doi:10.1002/jlcr.3973. ; Aslani A, Snowdon GM, Bailey DL, et al. Lutetium-177 DOTATATE production with an automated radiopharmaceutical synthesis system. Asia Ocean J Nucl Med Biol. 2015;3(2):107-115. ; Eryilmaz K, Kilbas B. Fully-automated synthesis of 177Lu labelled FAPI derivatives on the module modular lab-Eazy. EJNMMI Radiopharm Chem. 2021;6(1):16. doi:10.1186/s41181-021-00130-3. ; Wichmann CW, Ackermann U, Poniger S, et al. Automated radiosynthesis of [68Ga]Ga-PSMA-11 and [177Lu]Lu-PSMA-617 on the iPHASE MultiSyn module for clinical applications. J Label Compd Radiopharm. 2021;64(3):140-146. doi:10.1002/jlcr.3889. ; Garcia-Arguello SF, Lopez-Lorenzo B, Ruiz-Cruces R. Automated production of [68Ga]Ga-DOTANOC and [68Ga]Ga-PSMA-11 using a TRACERlab FXFN synthesis module. J Label Compd Radiopharm. 2019;62(3):146-153. doi:10.1002/jlcr.3706. ; Kelly JM, Amor-Coarasa A, Sweeney E, Wilson JJ, Causey PW, Babich JW. A suitable time point for quantifying the radiochemical purity of 225Ac-labeled radiopharmaceuticals. EJNMMI Radiopharm Chem. 2021;6(1):38. ; Kasliwal R. Product quality considerations in actinium-225 radiopharmaceuticals. US Food and Drug Administration 2021. Accessed November 7, 2023. https://fda.gov/media/152472/download. ; Hooijman EL, Chalashkan Y, Ling SW, et al. Development of [225Ac]Ac-PSMA-I&T for targeted alpha therapy according to GMP guidelines for treatment of mCRPC. Pharmaceutics. 2021;13(5):715. doi:10.3390/pharmaceutics13050715. ; Karmen A, Malikin G, Freundlich L, Lam S. High-sensitivity radioassay of chromatographic effluents: automatic fraction collector/concentrator for quantitative autoradiography. J Chromatogr A. 1985;349(2):267-274. doi:10.1016/S0021-9673(01)83782-1. Contributed Indexing: Keywords: 177Lu-PSMA-617; 225Ac-PSMA-617; HPLC; automated radiolabeling; module; radiosynthesis; robotic pipettor Substance Nomenclature: NTM296JU95 (Zirconium-89) ; 0 (Radioisotopes) ; 0 (PSMA-617) ; 0 (Actinium-225) ; NIK1K0956U (Actinium) ; C6V6S92N3C (Zirconium) ; 0 (Dipeptides) ; 0 (Heterocyclic Compounds, 1-Ring) ; EC 3.4.21.77 (Prostate-Specific Antigen) Entry Date(s): Date Created: 20240131 Date Completed: 20240305 Latest Revision: 20240305 Update Code: 20240305

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Automated radiolabeling and handling of <superscript>177</superscript> Lu- and <superscript>225</superscript> Ac-PSMA-617 using a robotic pipettor.