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Neonatal oxygen deficiency in rats may disturb growth and long-term metabolic homeostasis. In order to facilitate metabolic evaluation, the subjects are usually housed individually. However, social isolation associated with individually housed conditions alters animal behavior, which may influence the experimental results. This study investigated the effects of social isolation on neonatal anoxia-induced changes in growth and energy metabolism. Male and female Wistar rats were exposed, on postnatal day 2 (P2), to either 25-min of anoxia or control treatment. From P27 onward, part of the subjects of each group was isolated in standard cages, and the remaining subjects were housed in groups. At P34 or P95, the subjects were fasted for 18 h, refeed for 1 h, and then perfused 30 min later. Glycemia, leptin, insulin, and morphology of the pancreas were evaluated at both ages. For subjects perfused at P95, body weight and food intake were recorded up to P90, and the brain was collected for Fos and NeuN immunohistochemistry. Results showed that male rats exposed to neonatal anoxia and social isolation exhibited increased body weight gain despite the lack of changes in food intake. In addition, social isolation (1) decreased post-fasting weight loss and post-fasting food intake and (2) increased glycemia, insulin, and leptin levels of male and female rats exposed to anoxia and control treatments, both at P35 and P95. Furthermore, although at P35, anoxia increased insulin levels of males, it decreased the area of the β-positive cells in the pancreas of females. At P95, anoxia increased post-prandial weight loss of males, post-fasting food intake, insulin, and leptin, and decreased Fos expression in the arcuate nucleus (ARC) of males and females. Hyperphagia was associated with possible resistance to leptin and insulin, suspected by the high circulating levels of these hormones and poor neuronal activation of ARC. This study demonstrated that continuous social isolation from weaning modifies, in a differentiated way, the long-term energy metabolism and growth of male and female Wistar rats exposed to neonatal anoxia or even control treatments. Therefore, social isolation should be considered as a factor that negatively influences experimental results and the outcomes of the neonatal injury. These results should also be taken into account in clinical procedures, since the used model simulates the preterm babies' conditions and some therapeutic approaches require isolation.

Titel:	Post-weaning social isolation modifies neonatal anoxia-induced changes in energy metabolism and growth of rats.
Autor/in / Beteiligte Person:	Cruz-Ochoa, NA ; Motta-Teixeira, LC ; Cruz-Ochoa, PF ; Lopez-Paredes, S ; Ochoa-Amaya, JE ; Takada, SH ; Xavier, GF ; Nogueira, MI
Link:	Full Text Finder (Volltext)
Zeitschrift:	International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Jg. 84 (2024-06-01), Heft 4, S. 293-304
Veröffentlichung:	2020- : Hoboken, NJ : John Wiley & Sons, Inc. ; <i>Original Publication</i>: Oxford : New York : Pergamon Press, c1983-, 2024
Medientyp:	academicJournal
ISSN:	1873-474X (electronic)
DOI:	10.1002/jdn.10327
Schlagwort:	Animals Male Female Rats Leptin blood Leptin metabolism Blood Glucose metabolism Insulin blood Insulin metabolism Weaning Age Factors Social Isolation psychology Rats, Wistar Animals, Newborn Energy Metabolism physiology Eating physiology Hypoxia metabolism Body Weight physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Int J Dev Neurosci] 2024 Jun; Vol. 84 (4), pp. 293-304. <i>Date of Electronic Publication: </i>2024 Mar 26. MeSH Terms: Social Isolation* / psychology ; Rats, Wistar* ; Animals, Newborn* ; Energy Metabolism* / physiology ; Eating* / physiology ; Hypoxia* / metabolism ; Body Weight* / physiology ; Animals ; Male ; Female ; Rats ; Leptin / blood ; Leptin / metabolism ; Blood Glucose / metabolism ; Insulin / blood ; Insulin / metabolism ; Weaning ; Age Factors References: Adstamongkonkul, D., & Hess, D. C. (2017). Ischemic conditioning and neonatal hypoxic ischemic encephalopathy: A literature review. Conditioning Medicine, 1, 9–16. ; Bertile, F., & Raclot, T. (2006). The melanocortin system during fasting. Peptides, 27, 291–300. https://doi.org/10.1016/j.peptides.2005.03.063. ; Blencowe, H., Cousens, S., Oestergaard, M. Z., Chou, D., Moller, A. B., Narwal, R., Adler, A., Vera Garcia, C., Rohde, S., Say, L., & Lawn, J. E. (2012). National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries: A systematic analysis and implications. Lancet, 379, 2162–2172. https://doi.org/10.1016/S0140-6736(12)60820-4. ; Bullitt, E. (1990). Expression of C‐fos‐like protein as a marker for neuronal activity following noxious stimulation in the rat. The Journal of Comparative Neurology, 296, 517–530. https://doi.org/10.1002/cne.902960402. ; Caixeta, D. C., Teixeira, R. R., Peixoto, L. G., Machado, H. L., Baptista, N. B., De Souza, A. V., Vilela, D. D., Franci, C. R., & Salmen Espindola, F. (2018). Adaptogenic potential of royal jelly in liver of rats exposed to chronic stress. PLoS ONE, 13, e0191889. https://doi.org/10.1371/journal.pone.0191889. ; Challet, E., Le Maho, Y., Robin, J. P., Malan, A., & Cherel, Y. (1995). Involvement of corticosterone in the fasting‐induced rise in protein utilization and locomotor activity. Pharmacology, Biochemistry, and Behavior, 50, 405–412. https://doi.org/10.1016/0091-3057(94)00287-S. ; Chen, Y., & Baram, T. Z. (2016). Toward understanding how early‐life stress reprograms cognitive and emotional brain networks. Neuropsychopharmacology, 41, 197–206. https://doi.org/10.1038/npp.2015.181. ; Crujeiras, A. B., Carreira, M. C., Cabia, B., Andrade, S., Amil, M., & Casanueva, F. F. (2015). Leptin resistance in obesity: An epigenetic landscape. Life Sciences, 140, 57–63. https://doi.org/10.1016/j.lfs.2015.05.003. ; Cruz‐Ochoa, N. A., Ochoa‐Amaya, J. E., Pulecio, S. L., Xavier, G. F., & Nogueira, M. I. (2019). Neonatal anoxia impairs long‐term energy metabolism and somatic development of Wistar rats. International Journal of Developmental Neuroscience, 79, 76–85. https://doi.org/10.1016/j.ijdevneu.2019.11.001. ; Doucet, E., & Tremblay, A. (1997). Food intake, energy balance and body weight control. European Journal of Clinical Nutrition, 51, 846–855. https://doi.org/10.1038/sj.ejcn.1600497. ; Elias, C. F., Aschkenasi, C., Lee, C., Kelly, J., Ahima, R. S., Bjorbæk, C., Flier, J. S., Saper, C. B., & Elmquist, J. K. (1999). Leptin differentially regulates NPY and POMC neurons projecting to the lateral hypothalamic area. Neuron, 23, 775–786. https://doi.org/10.1016/S0896-6273(01)80035-0. ; Elmquist, J. K., Elias, C. F., & Saper, C. B. (1999). From lesions to leptin: Hypothalamic control of food intake and body weight. Neuron, 22, 221–232. https://doi.org/10.1016/S0896-6273(00)81084-3. ; Fonzo, G. A., Ramsawh, H. J., Flagan, T. M., Simmons, A. N., Sullivan, S. G., Allard, C. B., Paulus, M. P., & Stein, M. B. (2016). Early life stress and the anxious brain: Evidence for a neural mechanism linking childhood emotional maltreatment to anxiety in adulthood. Psychological Medicine, 45, 1037–1054. https://doi.org/10.1017/S0033291715002603. ; Frederich, R. C., Löllmann, B., Hamann, A., Napolitano‐Rosen, A., Kahn, B. B., Lowell, B. B., & Flier, J. S. (1995). Expression of ob mRNA and its encoded protein in rodents. Impact of nutrition and obesity. The Journal of Clinical Investigation, 96, 1658–1663. https://doi.org/10.1172/JCI118206. ; Gopagondanahalli, K. R., Li, J., Fahey, M. C., Hunt, R. W., Jenkin, G., Miller, S. L., & Malhotra, A. (2016). Preterm hypoxic‐ischemic encephalopathy. Frontiers in Pediatrics, 4, 1–10. ; Grojean, S., Schroeder, H., Pourié, G., Charriaut‐Marlangue, C., Koziel, V., Desor, D., Vert, P., & Daval, J. L. (2003). Histopathological alterations and functional brain deficits after transient hypoxia in the newborn rat pup: A long term follow‐up. Neurobiology of Disease, 14, 265–278. https://doi.org/10.1016/S0969-9961(03)00082-2. ; Grundwald, N. J., & Brunton, P. J. (2015). Prenatal stress programs neuroendocrine stress responses and affective behaviors in second generation rats in a sex‐dependent manner. Psychoneuroendocrinology, 62, 204–216. https://doi.org/10.1016/j.psyneuen.2015.08.010. ; Keesom, S. M., Morningstar, M. D., Sandlain, R., Wise, B. M., & Hurley, L. M. (2018). Social isolation reduces serotonergic fiber density in the inferior colliculus of female, but not male, mice. Brain Research, 1694, 94–103. https://doi.org/10.1016/j.brainres.2018.05.010. ; Kohlhauser, C., Kaehler, S., Mosgoeller, W., Singewald, N., Kouvelas, D., Prast, H., Hoeger, H., & Lubec, B. (1999). Histological changes and neurotransmitter levels three months following perinatal asphyxia in the rat. Life Sciences, 64, 2109–2124. https://doi.org/10.1016/S0024-3205(99)00160-5. ; Kumar, A. J., Takada, S. H., Motta‐Teixeira, L. C., Lee, V. Y., Xavier, G. F., & Nogueira, M. I. (2017). Sex differences in somatic and sensory motor development after neonatal anoxia in Wistar rats. Behavioural Brain Research, 333, 242–250. https://doi.org/10.1016/j.bbr.2017.07.009. ; Kurinczuk, J. J., White‐Koning, M., & Badawi, N. (2010). Epidemiology of neonatal encephalopathy and hypoxic‐ischaemic encephalopathy. Early Human Development, 86, 329–338. https://doi.org/10.1016/j.earlhumdev.2010.05.010. ; Liu, L., Oza, S., Hogan, D., Chu, Y., Perin, J., Zhu, J., Lawn, J. E., Cousens, S., Mathers, C., & Black, R. E. (2016). Global, regional, and national causes of under‐5 mortality in 2000–15: An updated systematic analysis with implications for the sustainable development goals. Lancet, 388, 3027–3035. https://doi.org/10.1016/S0140-6736(16)31593-8. ; Luna‐Illades, C., Morales, T., & Miranda‐anaya, M. (2017). Decreased food anticipatory activity of obese mice relates to hypothalamic c‐Fos expression. Physiology & Behavior, 179, 9–15. https://doi.org/10.1016/j.physbeh.2017.05.020. ; Matsuda, V. D. V., Tejada, M. B., Motta‐Teixeira, L. C., Ikebara, J. M., Cardoso, D. S., Machado‐Nils, A. V., Lee, V. Y., Diccini, I., Arruda, B. P., Martins, P. P., Dias, N. M. M., Tessarotto, R. P., Raeisossadati, R., Bruno, M., Takase, L. F., Kihara, A. H., Nogueira, M. I., Xavier, G. F., & Takada, S. H. (2021). Impact of neonatal anoxia and hypothermic treatment on development and memory of rats. Experimental Neurology, 340, 113691. https://doi.org/10.1016/j.expneurol.2021.113691. ; Morgan, J. I., Cohen, D. R., Hempstead, J. L., & Curran, T. O. M. (1987). Mapping patterns of c‐fos expression in the central nervous system after seizure. Science, 237, 192–197. https://doi.org/10.1126/science.3037702. ; Mumtaz, F., Khan, M. I., Zubair, M., & Dehpour, A. R. (2018). Neurobiology and consequences of social isolation stress in animal model—A comprehensive review. Biomedicine & Pharmacotherapy, 105, 1205–1222. https://doi.org/10.1016/j.biopha.2018.05.086. ; Myers, M. G., Leibel, R. L., Seeley, R. J., & Schwartz, M. W. (2010). Obesity and leptin resistance: Distinguishing cause from effect. Trends in Endocrinology and Metabolism, 21, 643–651. https://doi.org/10.1016/j.tem.2010.08.002. ; Nagashima, K., Nakai, S., Matsue, K., Konishi, M., Tanaka, M., & Kanosue, K. (2003). Effects of fasting on thermoregulatory processes and the daily oscillations in rats. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 284, R1486–R1493. https://doi.org/10.1152/ajpregu.00515.2002. ; Overton, J. M., & Williams, T. D. (2004). Behavioral and physiologic responses to caloric restriction in mice. Physiology & Behavior, 81, 749–754. https://doi.org/10.1016/j.physbeh.2004.04.025. ; Paxinos, G., & Watson, C. (1988). The rat BRAIN in stereotaxic coordinates (FOURTH EDITION CD‐ROM ed., Vol. 4). Academic Press. ; Pérez‐Cerezales, S., Ramos‐Ibeas, P., Rizos, D., Lonergan, P., Bermejo‐Alvarez, P., & Gutiérrez‐Adán, A. (2018). Early sex‐dependent differences in response to environmental stress. Reproduction, 155, R39–R51. https://doi.org/10.1530/REP-17-0466. ; Rezai‐Zadeh, K., Yu, S., Jiang, Y., Laque, A., Schwartzenburg, C., Morrison, C. D., Derbenev, A. V., Zsombok, A., & Münzberg, H. (2014). Leptin receptor neurons in the dorsomedial hypothalamus are key regulators of energy expenditure and body weight, but not food intake. Molecular Metabolism, 3, 681–693. https://doi.org/10.1016/j.molmet.2014.07.008. ; Sawchenko, P. E. (1998). Toward a new neurobiology of energy balance, appetite, and obesity. The Journal of Comparative Neurology, 442, 435–441. ; Schwartz, M. W., Seeley, R. J., Campfield, L. A., Burn, P., & Baskin, D. G. (1996). Identification of targets of leptin action in rat hypothalamus. The Journal of Clinical Investigation, 96, 1101–1106. https://doi.org/10.1172/JCI118891. ; Shimomura, C., & Ohta, H. (1988). Behavioral abnormalities and seizure susceptibility in rat after neonatal anoxia. Brain & Development, 10, 160–163. https://doi.org/10.1016/S0387-7604(88)80020-2. ; Syed, S. A., & Nemeroff, C. B. (2017). Early life stress, mood, and anxiety disorders. Chronic Stress, 1, 247054701769446. https://doi.org/10.1177/2470547017694461. ; Takada, S. H., Motta‐Teixeira, L. C., Machado‐Nils, A. V., Lee, V. Y., Sampaio, C. A., Polli, R. S., Malheiros, J. M., Takase, L. F., Kihara, A. H., Covolan, L., Xavier, G. F., & Nogueira, M. I. (2016). Impact of neonatal anoxia on adult rat hippocampal volume, neurogenesis and behavior. Behavioural Brain Research, 296, 331–338. https://doi.org/10.1016/j.bbr.2015.08.039. ; Takada, S. H., Sampaio, C. A. G., Allemandi, W., Ito, P. H., Takase, L. F., & Nogueira, M. I. (2011). A modified rat model of neonatal anoxia: Development and evaluation by pulseoximetry, arterial gasometry and Fos immunoreactivity. Journal of Neuroscience Methods, 198, 62–69. https://doi.org/10.1016/j.jneumeth.2011.03.009. ; Tang, A. C., & Nakazawa, M. (2005). Neonatal novelty exposure ameliorates anoxia‐induced hyperactivity in the open field. Behavioural Brain Research, 163, 1–9. https://doi.org/10.1016/j.bbr.2005.03.025. ; van Swieten, M. M. H., Pandit, R., Adan, R. A. H., & van der Plasse, G. (2014). The neuroanatomical function of leptin in the hypothalamus. Journal of Chemical Neuroanatomy, 61, 207–220. https://doi.org/10.1016/j.jchemneu.2014.05.004. ; Vannucci, R. C. (2000). Hypoxic ischemic encephalopathy. American Journal of Perinatology, 17, 451–464. https://doi.org/10.1055/s-2000-9293. ; Vargas, J., Junco, M., Gomez, C., & Lajud, N. (2016). Early life stress increases metabolic risk, HPA axis reactivity, and depressive‐like behavior when combined with postweaning social isolation in rats. PLoS ONE, 11, e0162665. https://doi.org/10.1371/journal.pone.0162665. ; Vargas, V. E., Gurung, S., Grant, B., Hyatt, K., Singleton, K., Myers, S. M., Saunders, D., Njoku, C., Towner, R., & Myers, D. A. (2017). Gestational hypoxia disrupts the neonatal leptin surge and programs hyperphagia and obesity in male offspring in the Sprague‐Dawley rat. PLoS ONE, 12, e0185272. https://doi.org/10.1371/journal.pone.0185272. ; Viana Borges, J., Souza de Freitas, B., Antoniazzi, V., de Souza dos Santos, C., Vedovelli, K., Naziaseno Pires, V., Paludo, L., Martins de Lima, M. N., & Bromberg, E. (2019). Social isolation and social support at adulthood affect epigenetic mechanisms, brain‐derived neurotrophic factor levels and behavior of chronically stressed rats. Behavioural Brain Research, 366, 36–44. https://doi.org/10.1016/j.bbr.2019.03.025. ; Yager, J. Y., & Ashwal, S. (2009). Animal models of perinatal hypoxic‐ischemic brain damage. Pediatric Neurology, 40, 156–167. https://doi.org/10.1016/j.pediatrneurol.2008.10.025. ; Yam, K. Y., Naninck, E. F. G., Schmidt, M. V., Lucassen, P. J., & Korosi, A. (2015). Early‐life adversity programs emotional functions and the neuroendocrine stress system: The contribution of nutrition, metabolic hormones and epigenetic mechanisms. Stress, 18, 328–342. https://doi.org/10.3109/10253890.2015.1064890. ; Yamada, K., Ohki‐Hamazaki, H., & Wada, K. (2000). Differential effects of social isolation upon body weight, food consumption, and responsiveness to novel and social environment in bombesin receptor subtype‐3 (BRS‐3) deficient mice. Physiology & Behavior, 68, 555–561. https://doi.org/10.1016/S0031-9384(99)00214-0. ; Yan, F., Zhang, M., Meng, Y., Li, H., Yu, L., Fu, X., Tang, Y., & Jiang, C. (2016). Erythropoietin improves hypoxic‐ischemic encephalopathy in neonatal rats after short‐term anoxia by enhancing angiogenesis. Brain Research, 1651, 104–113. https://doi.org/10.1016/j.brainres.2016.09.024. Grant Information: 2015/18415-8 Fundação de Amparo à Pesquisa do Estado de São Paulo; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Coordenadoria de Aperfeiçoamento de Pessoal de Nivel Superior Contributed Indexing: Keywords: anoxia; arcuate nucleus; food intake; insulin; leptin; social isolation Substance Nomenclature: 0 (Leptin) ; 0 (Blood Glucose) ; 0 (Insulin) Entry Date(s): Date Created: 20240326 Date Completed: 20240604 Latest Revision: 20240604 Update Code: 20240604

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Post-weaning social isolation modifies neonatal anoxia-induced changes in energy metabolism and growth of rats.