Subcutaneous injection of adipose stromal cell-secretome improves renal function and reduces inflammation in established acute kidney injury.
In: Stem cell research & therapy, Jg. 15 (2024-04-24), Heft 1, S. 119
Online
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Zugriff:
Background: Adipose stromal cells (ASC) are a form of mesenchymal stromal cells that elicit effects primarily via secreted factors, which may have advantages for the treatment of injury or disease. Several previous studies have demonstrated a protective role for MSC/ASC on mitigating acute kidney injury but whether ASC derived factors could hasten recovery from established injury has not been evaluated.
Methods: We generated a concentrated secretome (CS) of human ASC under well-defined conditions and evaluated its ability to improve the recovery of renal function in a preclinical model of acute kidney injury (AKI) in rats. 24 h following bilateral ischemia/reperfusion (I/R), rats were randomized following determination of plasma creatinine into groups receiving vehicle -control or ASC-CS treatment by subcutaneous injection (2 mg protein/kg) and monitored for evaluation of renal function, structure and inflammation.
Results: Renal function, assessed by plasma creatinine levels, recovered faster in ASC-CS treated rats vs vehicle. The most prominent difference between the ASC-CS treated vs vehicle was observed in rats with the most severe degree of initial injury (P cr > 3.0 mg/dl 24 h post I/R), whereas rats with less severe injury (P cr < 2.9 mg/dl) recovered quickly regardless of treatment. The quicker recovery of ASC-treated rats with severe injury was associated with less tissue damage, inflammation, and lower plasma angiopoietin 2. In vitro, ASC-CS attenuated the activation of the Th17 phenotype in lymphocytes isolated from injured kidneys.
Conclusions: Taken together, these data suggest that ASC-CS represents a potent therapeutic option to improve established AKI.
(© 2024. The Author(s).)
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Subcutaneous injection of adipose stromal cell-secretome improves renal function and reduces inflammation in established acute kidney injury.
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Autor/in / Beteiligte Person: | Ullah, MM ; Collett, JA ; Monroe, JC ; Traktuev, D ; Coleman, M ; March, KL ; Basile, DP |
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Zeitschrift: | Stem cell research & therapy, Jg. 15 (2024-04-24), Heft 1, S. 119 |
Veröffentlichung: | London : BioMed Central, 2024 |
Medientyp: | academicJournal |
ISSN: | 1757-6512 (electronic) |
DOI: | 10.1186/s13287-024-03736-x |
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