Effects of monovalent and divalent cations on the rheology of entangled DNA.
In: Soft matter, Jg. 20 (2024-05-15), Heft 19, S. 3980-3986
Online
academicJournal
Zugriff:
In this paper we investigate the effects of varying cation valency and concentration on the rheology of entangled λ DNA solutions. We show that monovalent cations moderately increase the viscoelasticty of the solutions mainly by stabilising linear concatenation of λ DNA "monomers" via hybridisation of their sticky ends. On the contrary, divalent cations have a far more complex and dramatic effect on the rheology of the solution and we observe evidence of inter-molecular DNA-DNA bridging by Mg 2+ . We argue that these results may be interesting in the context of dense solutions of single and double stranded DNA, e.g. in vivo or in biotechnology applications such as DNA origami and DNA hydrogels.
Titel: |
Effects of monovalent and divalent cations on the rheology of entangled DNA.
|
---|---|
Autor/in / Beteiligte Person: | Harnett, J ; Weir, S ; Michieletto, D |
Link: | |
Zeitschrift: | Soft matter, Jg. 20 (2024-05-15), Heft 19, S. 3980-3986 |
Veröffentlichung: | Cambridge, England : Royal Society of Chemistry, 2005-, 2024 |
Medientyp: | academicJournal |
ISSN: | 1744-6848 (electronic) |
DOI: | 10.1039/d3sm00957b |
Schlagwort: |
|
Sonstiges: |
|