Characterization of purification-associated reduction in IgE-dependent histamine release from rat peritoneal mast cells.
In: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Jg. 44 (1995-12-01), Heft 12, S. 541-7
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Zugriff:
Histamine release from purified rat peritoneal mast cells (PMC) was examined and compared to that from a non-purified preparation (PEC). Both PEC and PMC released similar amounts of histamine upon stimulation with compound 48/80, calcium ionophore A23187 and substance P. In contrast, IgE-dependent histamine release from PMC caused by antigen, anti-IgE and concanavalin A was very low compared to that of PEC. The reduced IgE-dependent histamine release from PMC, however, was recovered when PMC was reconstituted with non-mast cells (NMC) present in the peritoneal cavity. The effect was time-dependent and reached a plateau in 30 min. NMC from both sensitized and non-sensitized rats recovered the reduced histamine release from PMC dose-dependently. The potentiating effect of NMC was observed even in the presence of excess amount of phosphatidylserine. Supernatants of NMC and a mixture of PMC and NMC incubated for 1 hr at 37 degrees C, however, failed to potentiate the histamine release. These results demonstrate that IgE-dependent histamine release from rat peritoneal mast cells is upregulated by other cells present in the peritoneal cavity, and that the mechanism involved is distinct from that of phosphatidylserine.
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Characterization of purification-associated reduction in IgE-dependent histamine release from rat peritoneal mast cells.
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Autor/in / Beteiligte Person: | Inagaki, N ; Kawasaki, H ; Nagai, H |
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Zeitschrift: | Inflammation research : official journal of the European Histamine Research Society ... [et al.], Jg. 44 (1995-12-01), Heft 12, S. 541-7 |
Veröffentlichung: | Basel, Switzerland : Birkhäuser, c1995-, 1995 |
Medientyp: | academicJournal |
ISSN: | 1023-3830 (print) |
DOI: | 10.1007/BF01757359 |
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