GSH protects against oxidative stress and toxicity in VL-17A cells exposed to high glucose.
In: European Journal of Nutrition, Jg. 54 (2015-03-01), Heft 2, S. 223-234
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academicJournal
Zugriff:
Purpose: The deficiency of glutathione (GSH) has been linked to several diseases. The study investigated the role of GSH as a protective factor against hyperglycemia-mediated injury in VL-17A cells treated with 50 mM glucose. Methods: The cell viability and different oxidative stress parameters including glyoxalase I activity were measured. Results: GSH supplementation with 2 mM N-acetyl cysteine (NAC) or 0.1 mM ursodeoxycholic acid (UDCA) increased the viability, GSH level and the GSH-dependent glyoxalase I activity in 50 mM glucose-treated VL-17A cells. Further, pretreatment of 50 mM glucose-treated VL-17A cells with NAC or UDCA decreased oxidative stress (levels of reactive oxygen species and protein carbonylation), apoptosis (caspase 3 activity and annexin V-propidium iodide positive cells) and glutathionylated protein formation, a measure of oxidative stress. GSH depletion with 0.4 mM buthionine sulfoximine (BSO) or 1 mM diethyl maleate (DEM) potentiated the decrease in viability, glyoxalase I activity and increase in oxidative stress and apoptosis, with decreased GSH levels in 50 mM glucose-treated VL-17A cells. Conclusion: Thus, changes in GSH levels with exogenous agents such as NAC, UDCA, BSO or DEM modulate hyperglycemia-mediated injury in a cell model of VL-17A liver cells. [ABSTRACT FROM AUTHOR]
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Titel: |
GSH protects against oxidative stress and toxicity in VL-17A cells exposed to high glucose.
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Autor/in / Beteiligte Person: | Kumar, S. ; Swaminathan, Kavitha ; Clemens, Dahn ; Dey, Aparajita |
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Zeitschrift: | European Journal of Nutrition, Jg. 54 (2015-03-01), Heft 2, S. 223-234 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 1436-6207 (print) |
DOI: | 10.1007/s00394-014-0703-2 |
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