An ultrasensitive detection of 17β-estradiol using a gold nanoparticle-based fluorescence immunoassay.
In: Analyst, Jg. 140 (2015-03-15), Heft 6, S. 2001-2007
Online
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Zugriff:
A novel ultrasensitive amplification immunoassay for the determination of 17β-estradiol (E 2 ) is reported based on the nanoparticle signal amplification platform. It involves two types of particles: magnetic microparticles (MMPs) functionalized with an anti-E 2 antibody produced in rabbit as a capture probe; double-codified gold nanoparticles (DC-AuNPs) modified with both goat anti-rabbit antibody and SH-dsDNA-biotin as a signal amplifier; and avidin-FITC was added to link to the SH-dsDNA-biotin as a tracer. The competitive reaction of the anti-E 2 antibody immobilized on magnetic microparticles with estradiol in the sample solution and with the goat anti-rabbit antibody on double-codified gold nanoparticles results in a complex involving the DC-AuNPs and MMPs. Under optimized conditions, the linear range of E 2 is from 1.0 × 10 −5 to 1.0 ng mL −1 , and the detection limit of the assay could reach up to 6.37 × 10 −6 ng mL −1 . It was applied to determine E 2 in human urine, with mean percent recoveries in the range of 96.5%–107.4%, and relative standard deviations were below 8.1%. [ABSTRACT FROM AUTHOR]
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Titel: |
An ultrasensitive detection of 17β-estradiol using a gold nanoparticle-based fluorescence immunoassay.
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Autor/in / Beteiligte Person: | Du, Lingyun ; Ji, Wei ; Zhang, Yuanfu ; Zhang, Chunyan ; Liu, Guofu ; Wang, Shuhao |
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Zeitschrift: | Analyst, Jg. 140 (2015-03-15), Heft 6, S. 2001-2007 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 0003-2654 (print) |
DOI: | 10.1039/c4an01952k |
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