Mechanistic insight into beta-carotene-mediated protection against ulcerative colitis-associated local and systemic damage in mice.
In: European Journal of Nutrition, Jg. 54 (2015-06-01), Heft 4, S. 639-652
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Zugriff:
Purpose: Ulcerative colitis (UC), a chronic gastrointestinal disorder, is a debilitating disease affecting many people across the globe. Research suggests that the levels of several antioxidants, including β-carotene (β-CAR), decrease in the serum of patients with UC. The present study was aimed at elucidating the molecular mechanisms involved in β-CAR-mediated protection against UC in mice. Methods: UC was induced in mice using 3 %w/v dextran sulfate sodium in drinking water for two cycles; one cycle comprised of 7 days of dextran sulfate sodium-treated water followed by 14 days of normal drinking water. β-CAR was administered at the doses of 5, 10 and 20 mg/kg bw/day, po throughout the experiment. The effect of β-CAR in mice with UC was evaluated using biochemical parameters, histological evaluation, comet and micronucleus assays, immunohistochemistry and Western blot analysis. Results: The results indicated that β-CAR treatment ameliorated the severity of UC by modulating various molecular targets such as nuclear factor-kappa B, cyclooxygenase-2, interleukin 17, signal transducer and activator of transcription 3, nuclear erythroid 2-related factor 2, matrix metalloproteinase-9 and connective tissue growth factor. Further, β-CAR treatment maintained the gut integrity by increasing the expression of a tight junction protein, occludin, which was decreased in the colon of mice with UC. Also β-CAR treatment significantly reduced UC-associated elevated plasma lipopolysaccharide level, systemic inflammation and genotoxicity. Conclusion: β-CAR ameliorated UC-associated local and systemic damage in mice by acting on multiple targets. [ABSTRACT FROM AUTHOR]
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Titel: |
Mechanistic insight into beta-carotene-mediated protection against ulcerative colitis-associated local and systemic damage in mice.
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Autor/in / Beteiligte Person: | Trivedi, P. ; Jena, G. |
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Zeitschrift: | European Journal of Nutrition, Jg. 54 (2015-06-01), Heft 4, S. 639-652 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 1436-6207 (print) |
DOI: | 10.1007/s00394-014-0745-5 |
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