جدا سازی لیستریا مونوسیتوژنز از محصولات گوشتی و بررسی ژن prf A در باکتریهای جداسازی شده و مقایسه آن با نمونه های کلینیکی و نمونه استاندارد (Persian)

Backgrounds: Listeria is a gram positive, facultative intracellular bacteria. This study was to investigate Listeria monocytogenes in various meat products and presence of prf A gene in bacteria isolated from food samples and compare them with clinical and standard samples. Materials and Method: In this descriptive-analytical study, sixty samples of beef, chicken, ham, cocktail, and sausage were collected from Khoramabad slaughterhouse and shops in Khoramabad and Tehran. L. monocytogenes was isolated by cold enrichment method and presence of prfA gene was assessed by Polymerase Chain Reaction method. Results: From 60 samples of meat and meat products, 8 (%13.3) were positive for Listeria spp. Four samples (% 6.6) were L. monocytogenes, three (%5) were L. innocua and 1 sample (%1.6) was L. welshimeri. Listeria innocua was isolated from meat and poultry samples, L. monocytogens from meat, chicken, ham, and L. welshimeri was isolated from meat. Prf A gene was detected in isolated L. monocytogenes and donated bacteria from dairy products, clinical and standard samples. Two of donated samples of vegetable contained prf A genes. Conclusions: Listeria bacteria is transmitted through food and is a serious threat to public health. So by identification of bacteria and especially its genes, perhaps we find a way to prevent disease caused by the bacteria. [ABSTRACT FROM AUTHOR]

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