Differential Sensitivity of Target Genes to Translational Repression by miR-17~92.
In: PLoS Genetics, Jg. 13 (2017-02-27), Heft 2, S. 1-38
Online
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Zugriff:
MicroRNAs (miRNAs) are thought to exert their functions by modulating the expression of hundreds of target genes and each to a small degree, but it remains unclear how small changes in hundreds of target genes are translated into the specific function of a miRNA. Here, we conducted an integrated analysis of transcriptome and translatome of primary B cells from mutant mice expressing miR-17~92 at three different levels to address this issue. We found that target genes exhibit differential sensitivity to miRNA suppression and that only a small fraction of target genes are actually suppressed by a given concentration of miRNA under physiological conditions. Transgenic expression and deletion of the same miRNA gene regulate largely distinct sets of target genes. miR-17~92 controls target gene expression mainly through translational repression and 5’UTR plays an important role in regulating target gene sensitivity to miRNA suppression. These findings provide molecular insights into a model in which miRNAs exert their specific functions through a small number of key target genes. [ABSTRACT FROM AUTHOR]
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Titel: |
Differential Sensitivity of Target Genes to Translational Repression by miR-17~92.
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Autor/in / Beteiligte Person: | Jin, Hyun Yong ; Oda, Hiroyo ; Chen, Pengda ; Yang, Chao ; Zhou, Xiaojuan ; Kang, Seung Goo ; Valentine, Elizabeth ; Kefauver, Jennifer ; Liao, Lujian ; Zhang, Yaoyang ; Gonzalez-Martin, Alicia ; Shepherd, Jovan ; Morgan, Gareth J. ; Mondala, Tony S. ; Head, Steven R. ; Kim, Pyeung-Hyeun ; Xiao, Nengming ; Fu, Guo ; Liu, Wen-Hsien ; Han, Jiahuai |
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Zeitschrift: | PLoS Genetics, Jg. 13 (2017-02-27), Heft 2, S. 1-38 |
Veröffentlichung: | 2017 |
Medientyp: | academicJournal |
ISSN: | 1553-7390 (print) |
DOI: | 10.1371/journal.pgen.1006623 |
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