Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell.
In: Oncology Letters, Jg. 15 (2018-05-01), Heft 5, S. 7981-7986
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Zugriff:
The present study aimed to observe the effects of sulindac sulfide on the proliferation and apoptosis of human breast cancer cells MCF-7, and to explore the potential underlying molecular mechanism. The inhibitory ratio was detected using a cell counting kit-8 assay. The changes in cell cycle distribution were assessed using flow cytometry (FCM). Furthermore, the changes in cell apoptosis rates were detected by Hoechst 33258 staining and FCM coupled with Annexin V-FITC/propidium iodide (PI) staining. In addition, the protein expression was detected using western blotting. Sulindac sulfide was able to inhibit the proliferation of breast cancer in a dose- and time-dependent manner. In addition, sulindac sulfide altered the cell cycle of breast cancer cells. The results of Hoechst 33258 staining and FCM coupled with Annexin V-FITC/PI staining demonstrated that sulindac sulfide could significantly induce the apoptosis of MCF-7 cells in a dose-dependent, and time-dependent manner. The western blot analysis demonstrated the protein expression of Bcl-2 was downregulated, and Bax and cleaved caspase-3 were upregulated. The results of the present study suggest that sulindac sulfide can inhibit the proliferation and induce the apoptosis of MCF-7 cells. [ABSTRACT FROM AUTHOR]
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Titel: |
Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell.
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Autor/in / Beteiligte Person: | Sui, He-Huan ; Zhou, Yun-Jiang ; Wang, Hu ; Li, Li ; Cao, Min ; Huang, Jia-Jun |
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Zeitschrift: | Oncology Letters, Jg. 15 (2018-05-01), Heft 5, S. 7981-7986 |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 1792-1074 (print) |
DOI: | 10.3892/ol.2018.8331 |
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