HSP90: A Novel Target Gene of miRNA-628-3p in A549 Cells.
In: BioMed Research International, Jg. 2018 (2018-05-20), S. 1-10
Online
academicJournal
Zugriff:
Lung cancer is one of the leading causes of cancer-related death in the world. MicroRNA- (miR-) 628-3p plays critical roles in many cancers, including lung cancer. We investigated how miR-628-3p affected migration and apoptosis in A549 cells. We used bioinformatics algorithms to predict the miR-628-3p target gene to study the molecular mechanism by which miR-628-3p contributes to lung cancer. Then, we used the luciferase reporter assay to identify whether heat shock protein 90a (HSP90) is a direct target of miR-628-3p. Western blotting and quantitative real-time PCR showed that miR-628-3p downregulated HSP90a protein expression via a posttranscriptional mechanism. We confirm that miR-628-3p promotes apoptosis and inhibits migration in A549 cells by negatively regulating HSP90 . Our results may reveal a novel strategy for lung cancer treatment. [ABSTRACT FROM AUTHOR]
Copyright of BioMed Research International is the property of Hindawi Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
HSP90: A Novel Target Gene of miRNA-628-3p in A549 Cells.
|
---|---|
Autor/in / Beteiligte Person: | Pan, Jieli ; Jiang, Fusheng ; Zhou, Jia ; Wu, Dehong ; Sheng, Zhenhua ; Li, Meiya |
Link: | |
Zeitschrift: | BioMed Research International, Jg. 2018 (2018-05-20), S. 1-10 |
Veröffentlichung: | 2018 |
Medientyp: | academicJournal |
ISSN: | 2314-6133 (print) |
DOI: | 10.1155/2018/4149707 |
Schlagwort: |
|
Sonstiges: |
|