Expression of the E-selectin gene in human gingival epithelial tissue.
In: Journal of Oral Pathology & Medicine, Jg. 25 (1996-07-01), Heft 6, S. 320-324
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Zugriff:
E-selectin is an adhesion molecule, expressed by cytokine-activated endothelial cells, that participates in the binding of neutrophils. Recent studies in our laboratory documented binding of the E-selectin-specific monoclonal antibody H4/18 to keratinocytes in inflamed human oral mucosa, particularly gingival epithelium. To determine whether this immunoreactivity was due to expression of authentic E-selectin, the presence of E-selectin mRNA in gingival epithelium was analysed using the polymerase chain reaction (PCR). Reverse transcription of epithelial RNA and amplification of cDNA with E-selectin-specific primers resulted in the formation of a 178 nucleotide PCR product identical to that obtained from cytokine-activated endothelial cells. Sequencing of the PCR product revealed 100% homology between epithelial and endothelial E-selectin fragments. Epithelial preparations did not contain mRNA for von Willebrand factor, excluding the possibility of contamination by endothelial cells. These results confirm immunohistochemical studies of E-selectin immunoreactivity in human oral mucosa and demonstrate that E-selectin expression is not confined to endothelium. [ABSTRACT FROM AUTHOR]
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Titel: |
Expression of the E-selectin gene in human gingival epithelial tissue.
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Autor/in / Beteiligte Person: | Pietrzak, Eva R. ; Savage, Nell W. ; Aldred, Michael J. ; Walsh, Laurence J. ; Pietrzak, E R ; Savage, N W ; Aldred, M J ; Walsh, L J |
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Zeitschrift: | Journal of Oral Pathology & Medicine, Jg. 25 (1996-07-01), Heft 6, S. 320-324 |
Veröffentlichung: | 1996 |
Medientyp: | academicJournal |
ISSN: | 0904-2512 (print) |
DOI: | 10.1111/j.1600-0714.1996.tb00270.x |
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