MIM1 induces COLO829 melanoma cell death through mitochondrial membrane breakdown, GSH depletion, and DNA damage.
In: Fundamental & Clinical Pharmacology, Jg. 34 (2020-02-01), Heft 1, S. 20-31
Online
academicJournal
Zugriff:
Malignant melanoma is a high aggressive malignancy in humans and causes 60–80% of deaths from skin cancer. Defect in an intrinsic pathway of apoptosis via overexpression of Mcl‐1 is responsible for malignant melanoma development and progression, and also for resistance to chemotherapeutic agents. MIM1 is a specific low molecular Mcl‐1 protein inhibitor that is able to induce Mcl‐1‐dependent cancer cells death. Here, we examined the effect of MIM1 as well as MIM1 and dacarbazine (DTIC) mixture on cell viability, apoptosis, and cell cycle progression in COLO829 melanoma cells. Cell viability was performed by the WST‐1 assay. Analysis of apoptosis as well as cell cycle progression was determined by fluorescence image cytometer NucleoCounter NC‐3000. The obtained results demonstrated that the MIM1 exhibited high cytotoxicity against melanotic melanoma cells and induced mitochondrial membrane breakdown, GSH depletion, and DNA fragmentation. Additionally, MIM1 enhanced the proapoptotic effect of DTIC toward melanoma cells; furthermore, a mixture of these drugs caused cell cycle arrest at G2/M phase in COLO829 cells. Taken together, these data provide, for the first time, evidence that a low molecular weight Mcl‐1 inhibitor—MIM1 may be a promising agent with antitumor and proapoptotic properties toward melanoma cells. [ABSTRACT FROM AUTHOR]
Copyright of Fundamental & Clinical Pharmacology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
MIM1 induces COLO829 melanoma cell death through mitochondrial membrane breakdown, GSH depletion, and DNA damage.
|
---|---|
Autor/in / Beteiligte Person: | Respondek, Michalina ; Beberok, Artur ; Rzepka, Zuzanna ; Rok, Jakub ; Wrześniok, Dorota |
Link: | |
Zeitschrift: | Fundamental & Clinical Pharmacology, Jg. 34 (2020-02-01), Heft 1, S. 20-31 |
Veröffentlichung: | 2020 |
Medientyp: | academicJournal |
ISSN: | 0767-3981 (print) |
DOI: | 10.1111/fcp.12503 |
Schlagwort: |
|
Sonstiges: |
|