miR-98-5p protects against cerebral ischemia/reperfusion injury through anti-apoptosis and anti-oxidative stress in mice.
In: Journal of Biochemistry, Jg. 169 (2021-02-01), Heft 2, S. 195-206
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Zugriff:
Cerebral ischemia/reperfusion (I/R) injury is an obstacle in treating ischemic stroke effectively. miR-98-5p has been reported to have the ability of reducing myocardial I/R injury. To explore the function of miR-98-5p in cerebral I/R, we established mice model of middle cerebral artery occlusion and reperfusion (MCAO/R). The level of miR-98-5p was found to be downregulated in serum of stroke patients and brain tissues of MCAO/R mice. Examination of brain tissues indicated that upregulating miR-98-5p level alleviated the infarction in MCAO/R mice. Moreover, the upregulation of miR-98-5p reduced reactive oxygen species production and enhanced superoxide dismutase activity in brain tissues of MCAO/R mice. These results indicating that miR-98-5p could protect against oxidative stress. Further study showed that miR-98-5p inhibited apoptosis by reducing the levels of death-associated protein kinase 1, B cell lymphoma/leukaemia-2 associated x protein and cleaved caspase-3, as well as increasing the level of B cell lymphoma/leukaemia-2. In addition, miR-98-5p was found to protect against oxidative stress through downregulating the level of BTB domain and CNC homology 1 and upregulating the levels of NAD(P)H: quinone oxidoreductase 1 and heme oxygenase 1. Therefore, miR-98-5p might be a potential target to treat cerebral I/R injury. [ABSTRACT FROM AUTHOR]
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Titel: |
miR-98-5p protects against cerebral ischemia/reperfusion injury through anti-apoptosis and anti-oxidative stress in mice.
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Autor/in / Beteiligte Person: | Yu, Shan ; Zhai, Jingjie ; Yu, Jing ; Yang, Qiwei ; Yang, Jinghui |
Zeitschrift: | Journal of Biochemistry, Jg. 169 (2021-02-01), Heft 2, S. 195-206 |
Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
ISSN: | 0021-924X (print) |
DOI: | 10.1093/jb/mvaa099 |
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