Rapid generation of hybrid biochemical/mechanical cues in heterogeneous droplets for high-throughput screening of cellular responses.
In: Lab on a Chip, Jg. 21 (2021-07-21), Heft 14, S. 2691-2701
academicJournal
Zugriff:
Cells in their native microenvironment are subjected to varying combinations of biochemical cues and mechanical cues in a wide range. Although many signaling pathways have been found to be responsive for extracellular cues, little is known about how biochemical cues crosstalk with mechanical cues in a complex microenvironment. Here, we introduced heterogeneous droplets on a microchip, which were rapidly assembled by combining wettability-patterned microchip and programmed droplet manipulations, for a high-throughput cell screening of the varying combinations of biochemical cues and mechanical cues. This platform constructed a heterogeneous droplet/microgel array with orthogonal gradual chemicals and materials, which was further applied to analyze the cellular Wnt/β-catenin signaling in response to varying combinations of Wnt ligands and substrate stiffness. Thus, this device provides a powerful multiplexed bioassay platform for drug development, tissue engineering, and stem cell screening. [ABSTRACT FROM AUTHOR]
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Titel: |
Rapid generation of hybrid biochemical/mechanical cues in heterogeneous droplets for high-throughput screening of cellular responses.
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Autor/in / Beteiligte Person: | Zhao, Xing ; Ou, Gaozhi ; Lei, Mengcheng ; Zhang, Yang ; Li, Lina ; Ge, Anle ; Wang, Yachao ; Li, Yiwei ; Liu, Bi-Feng |
Zeitschrift: | Lab on a Chip, Jg. 21 (2021-07-21), Heft 14, S. 2691-2701 |
Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
ISSN: | 1473-0197 (print) |
DOI: | 10.1039/d1lc00209k |
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