基于代谢组学的虾夷扇贝不同组织风味与 营养成分变化差异. (Chinese)

In order to reveal the differences in the nutritional value and flavor of different genders and tissues of scallop (Patinopecten yessoensis), free amino acids, 5’-nucleotides and lipids in the samples were analyzed by targeted and nontargeted metabolomics based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and ultrahigh performance liquid chromatography-Q-Exactive Orbitrap tandem mass spectrometry (UPLC-Q-Exactive OrbitrapMS). Results showed that glutamic acid and glycine were the main free amino acids in scallop. The contents of glutamic acid and glycine in the adductor muscle were 132.98 and 280.37 μg/g, respectively and were little affected by gender. Adenosine 5’-monophosphate (AMP) was abundant in the adductor muscle and mantle, and the content of AMP in the adductor muscle of female scallops was about twice higher than that in male scallops. A total of 188 lipids were detected in the tissues of scallop, including phospholipids and glycerides. The content of phospholipids in the adductor muscle of male scallops was higher than that in female scallops, while the opposite was true for the content of phospholipids in the gonads. The digestive gland was an important part for storing glycerides, accounting for 49.36% and 33.11% of the total glycerides in male and female scallops, respectively. These results are of great significance for guiding the development of scallop products and the comprehensive processing and utilization of scallop by-products. [ABSTRACT FROM AUTHOR]

为研究虾夷扇贝 (Patinopecten yessoensis) 不同性别和不同组织的营养价值和风味差异, 使用液相色谱串联质谱和超高效液相色谱-Q-Exactive轨道阱串联质谱法对游离氨基酸、5’-核苷酸和脂质分别进行靶向与非靶 向代谢组学分析。研究显示, 谷氨酸和甘氨酸是虾夷扇贝主要的游离氨基酸, 闭壳肌中谷氨酸和甘氨酸含量高达 132.98 μg/g和280.37 μg/g, 性别对其影响较小。以腺嘌呤核糖核苷酸 (adenosine 5’-monophosphate, AMP) 为代表 的5’-核苷酸在闭壳肌和外套膜中含量较高, 雌性闭壳肌中的AMP约为雄性的2 倍。在虾夷扇贝不同组织中共检测到 188 种脂质, 主要为磷脂和甘油酯。雄性扇贝闭壳肌磷脂含量高于雌性, 而性腺中磷脂含量则与之相反。消化腺是 贮存甘油酯的重要部位, 在雌雄扇贝中分别占总甘油酯的49.36%和33.11%。研究结果对指导虾夷扇贝产品的开发 和副产物的综合加工利用有重要意义。 [ABSTRACT FROM AUTHOR]

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