Co-administration with A1M does not influence apoptotic response of <superscript>177</superscript>Lu-octreotate in GOT1 neuroendocrine tumors.
In: Scientific Reports, Jg. 13 (2023-04-19), Heft 1, S. 1-14
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Zugriff:
Recombinant α 1 -microglobulin (A1M) is a proposed radioprotector during 177 Lu-octreotate therapy of neuroendocrine tumors (NETs). To ensure a maintained therapeutic effect, we previously demonstrated that A1M does not affect the 177 Lu-octreotate induced decrease in GOT1 tumor volume. However, the underlying biological events of these findings are still unknown. The aim of this work was to examine the regulation of apoptosis-related genes in GOT1 tumors short-time after i.v. administration of 177 Lu-octreotate with and without A1M or A1M alone. Human GOT1 tumor-bearing mice received 30 MBq 177 Lu-octreotate or 5 mg/kg A1M or co-treatment with both. Animals were sacrificed after 1 or 7 days. Gene expression analysis of apoptosis-related genes in GOT1 tissue was performed with RT-PCR. In general, similar expression patterns of pro- and anti-apoptotic genes were found after 177 Lu-octreotate exposure with or without co-administration of A1M. The highest regulated genes in both irradiated groups compared to untreated controls were FAS and TNFSFRS10B. Administration of A1M alone only resulted in significantly regulated genes after 7 days. Co-administration of A1M did not negatively affect the transcriptional apoptotic response of 177 Lu-octreotate in GOT1 tumors. [ABSTRACT FROM AUTHOR]
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Co-administration with A1M does not influence apoptotic response of <superscript>177</superscript>Lu-octreotate in GOT1 neuroendocrine tumors.
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Autor/in / Beteiligte Person: | Rassol, Nishte ; Andersson, Charlotte ; Pettersson, Daniella ; Al-Awar, Amin ; Shubbar, Emman ; Kovács, Anikó ; Åkerström, Bo ; Gram, Magnus ; Helou, Khalil ; Forssell-Aronsson, Eva |
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Zeitschrift: | Scientific Reports, Jg. 13 (2023-04-19), Heft 1, S. 1-14 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/s41598-023-32091-9 |
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