盐胁迫盐芥和拟南芥褪黑素含量及合成相关基因表达 模式分析. (Chinese)

The halophyte Eutrema salsugineum has strong salt tolerance and the characteristics of model plants. It is an ideal material for studying the mechanism of plant coping with adversity. As a multifunctional hormone, the role of melatonin in the salt tolerance of E. salsugineum remains unclear. In this study, we performed the enzyme-linked immunosorbent assay of melatonin and real-time fluorescence quantitative PCR analysis. The accumulations of melatonin in different tissues, the synthesis of melatonin, the expression patterns of related genes and the effect of exogenous melatonin on the phenotype response to salt stress were analyzed in E. salsugineum and Arabidopsis. The results showed the melatonin synthesis in both plants were the highest in the young leaves, the endogenous melatonin of E. salsugineum was significantly higher than in Arabidopsis. The melatonin level was induced by salt stress in both plants, but the melatonin synthesis in Arabidopsis obviously decreased after salt stress for 7 d, which was different from the accumulation pattern in E. salsugineum. It was found that the genes related to melatonin synthesis in Arabidopsis and E. salsugineum were closely among different species of plants through sequence alignment analysis. Salt-responsive expression analysis showed that the expression abundance of SNAT1, ASMT and COMT were significantly up-regulated after salt stress for 3 d in E. salsugineum, which also induced by salt treatment for 1 d and 3 d in Arabidopsis, and then turned to down regulation under 7 d treatment; while there was no change in E. salsugineum, revealing that the expression changes of related genes upon salt condition in E. salsugineum and Arabidopsis was distinct. Furthermore, exogenous melatonin treatment significantly alleviated the stress phenotype of both plants under salt stress. Taken together, melatonin effectively contributed to the salt resistance of E. salsugineum and is involved in the regulation of salt tolerance of E. salsugineum and Arabidopsis, while the induction pattern of melatonin synthesis for them was different. [ABSTRACT FROM AUTHOR]

盐生植物盐芥（Eutrema salsugineum）耐盐适应性强且具备模式植物特征，是研究植物逆境适应机理的理想材料。 作为一种多功能的激素信号分子，褪黑素在盐芥耐盐性中的作用仍不清楚。本研究以盐芥为主要材料，以拟南芥做对比，主要通 过褪黑素酶联免疫以及实时荧光定量 PCR 分析，比较了二者在不同组织中褪黑素的积累和在响应盐胁迫过程中褪黑素合成、相关 基因的表达模式以及外源褪黑素处理对其盐应答表型的影响。结果显示，两种植物的褪黑素合成均在幼叶中最高，盐芥本底褪黑 素合成水平显著高于拟南芥，且盐胁迫诱导了两种植物中的褪黑素含量，但不同于盐芥，拟南芥在处理 7 d 后褪黑素合成明显下降。 通过序列比对发现在不同植物中，盐芥和拟南芥褪黑素合成相关基因的亲缘关系较近。盐应答表达分析显示，盐芥 SNAT1、ASMT 和 COMT 在盐处理 3 d 表达上调，而拟南芥中的相关基因在处理 1 d 和 3 d 后受盐诱导，7 d 后拟南芥中表达下降而盐芥中则无明 显变化，表明两种植物相关基因响应盐信号的表达变化存在差异。此外，外源褪黑素处理明显缓解了两种植物在盐逆境下的胁迫 表型。综上，褪黑素有效贡献于盐芥抗盐性，参与调节盐芥和拟南芥的耐盐适应过程，但二者对褪黑素合成的诱导模式不同。 [ABSTRACT FROM AUTHOR]

Copyright of Biotechnology Bulletin is the property of Biotechnology Bulletin Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)