Sputum leukotrienes in obstructive airways diseases.
In: Clinical & Experimental Immunology, Jg. 55 (1984-02-01), Heft 2, S. 397-404
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Zugriff:
Sputum samples from patients with bronchial asthma, chronic bronchitis and cystic fibrosis were examined for the presence of leukotrienes B 4 , C 4 and D 4 . Following ethanol extraction and purification on Amberlite XAD-8, leukotrienes were identified by high pressure liquid chromatography (HPLC) using the appropriate markers. Fractions from HLPC were also tested for biological activity using both the Boyden chemotaxis assay and FPL 55712 inhibitable contraction of the isolated guinea-pig ileum. LTB 4 was detected in the HPLC fractionated sputa from bronchial asthma (seven of seven), chronic bronchitis (four of four) and cystic fibrosis (four of four). In contrast, bioassay on the guinea-pig ileum failed to detect LTC 4 or LTD 4 in 17 asthmatic sputa, although they were detected in one of five bronchitics and 16 of 25 patients with cystic fibrosis. The activity in eight of these cystic fibrosis sputa was further characterized by HPLC and shown to be LTC 4 and/or LTD 4 . Sputum from 11 of 17 asthmatics, four of 25 patients with cystic fibrosis and two of five bronchitics contained an anaphylatoxin like substance. The majority of sputum samples containing LTB 4 also possessed an activity with physical and biological characteristics of the 5(S), 12(S), 6-trans LTB 4 isomer. These studies indicate that lipoxygenase products of arachidonic acid metabolism are present in the sputum in various forms of obstructive airways disease. The failure to detect the 'SRS-A' leukotrienes in sputum from bronchial asthma may be attributable to either losses during extraction, the insensitivity of the assay procedure or to more rapid catabolism of LTC 4 and LTD 4 by bronchial secretions in asthma than in cystic fibrosis. [ABSTRACT FROM AUTHOR]
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Sputum leukotrienes in obstructive airways diseases.
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Autor/in / Beteiligte Person: | O'Driscoll, B. R. C. ; Cromwell, O. ; Kay, A.B. |
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Zeitschrift: | Clinical & Experimental Immunology, Jg. 55 (1984-02-01), Heft 2, S. 397-404 |
Veröffentlichung: | 1984 |
Medientyp: | academicJournal |
ISSN: | 0009-9104 (print) |
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