酿酒葡萄铁调节转运蛋白基因 VvIRT1 的克隆、表达与 功能. (Chinese)

The genes VvIRT of iron regulated transporters（IRT）family from wine grape was cloned, its expression pattern and potential biological function were analyzed, which may provide theoretical foundation for Fe absorption and efficient utilization mechanisms of fruit trees. Homology cloning technology was used to clone the VvIRT1 gene from wine grape, real-time quantitative PCR was applied to analyze tissue-specific expression characteristics and their responses to different Fe supplies, and yeast heterologous expression system was to analyze the functional verification. The iron regulated transporter VvIRT1 from diploid wine grape ‘Marselan’ was isolated and determined. Phylogenetic tree analysis showed that VvIRT1 was closely clustered with citrus CsIRT1（Rutaceae）and upland cotton GhIRT1（Malvaceae）that the genetic distance among them was relatively close. VvIRT1 was specifically expressed in the roots of both 5-year-old mature trees and tissue-cultured seedlings. Fe depletion significantly induced the expressions of VvIRT1 in the whole tested seedlings, while high Fe toxicity significantly reduced the expressions of VvIRT1 in all tested seedlings in the roots of tissue-cultured seedlings. In addition, VvIRT1 recovered the normal growth of yeast mutant DEY1453 and had ability of transporting external Fe 2+ . [ABSTRACT FROM AUTHOR]

克隆酿酒葡萄铁调节转运蛋白基因 VvIRT，分析其表达模式及其潜在的生物学功能，为研究果树铁素吸收与高效利 用机制提供理论依据。利用同源克隆法克隆酿酒葡萄 VvIRT1，运用实时荧光定量 PCR 分析组织特异性表达模式及其在转录水平对 不同铁素供应水平的响应特征，借助酵母异源表达系统分析其转运 Fe2+ 的功能。从二倍体酿酒葡萄‘马瑟兰’中分离和鉴定了 1 个铁调节转运蛋白 VvIRT1，系统发育树表明 VvIRT1 与芸香科柑橘 CsIRT1 和锦葵科陆地棉 GhIRT1 的遗传距离较近。VvIRT1 在 5 年生成年树体新生根和组培幼苗根中特异表达 ；缺铁处理显著诱导了 VvIRT1 在组培幼苗全部组织（根、茎和叶）中表达，而高铁 毒害显著抑制了 VvIRT1 在组培幼苗根中的表达。此外，酵母功能互补试验表明，VvIRT1 能够恢复酵母突变菌株 DEY1453 的正常 生长，VvIRT1 具有转运外界 Fe 2+ 的能力。 [ABSTRACT FROM AUTHOR]

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