结直肠癌组织和细胞中DEAD盒解旋酶10 和Bystin 样蛋白的表达及其 临床意义. (Chinese)

Objective: To investigate the correlation between DEAD box RNA helicases 10 (DDX10) and Bystin-like（BYSL）in colorectal cancer (CRC) tissues and colon cancer DLD-1 cells and its clinical significance. Methods: A total of 78 pairs of CRC tissues and corresponding paracancerous tissues surgically resected in the Second Affiliated Hospital of Fujian Medical University from March 2017 to March 2018 were collected for this study. The expression levels of DDX10 and BYSL in the cancer and paracancerous tissues were detected by immunohistochemistry EnVision method.SiDDX10 and siNC were transfected into DLD-1 cells by transient transfection technology, respectively. The effect of DDX10 expression on BYSL in colorectal cancer cells was detected by PCR. CCK-8 and transwell assays were used to verify the effect of DDX10 and BYSL on proliferation, migration and invasion of colorectal cancer cells. Results: The immunohistochemistry EnVision results showed that the expression levels of DDX10 (73.08%) and BYSL (74.36%) were significantly higher in colorectal cancer tissues compared with paracancerous tissues. The results of chi-square test showed that the differences in DDX10 and BYSL expression were statistically significant in CRC patients with different tumor stages and different lymph node metastasis and recurrence status (all P<0.05). Pearson correlation analysis showed that DDX10 expression waspositively correlated with BYSL expression (r=0.636, P<0.001). Kaplan-Meier survival analysis showed that the high expression of DDX10 and BYSL was correlated with a worse prognosis of patients. Logistic regression analysis proved that DDX10 and BYSL were independent risk factors for CRC recurrence. The results of CCK-8 assay and Transwell assay showed that suppressing the expression of DDX10 could decrease the expression of BYSL and inhibit the proliferation, migration and invasion of colorectal cancer cells (all P<0.05). Conclusion: DDX10 and BYSL are highly expressed in CRC tissues, and they are positively associated with each other. Higher expression levels of DDX10 and BYSL are associated with poorer patient prognosis and were independent risk factors for CRC recurrence. Suppressing the expression of DDX10 can decrease the expression of BYSL, and inhibit the proliferation, migration and invasion of colorectal cancer cells. [ABSTRACT FROM AUTHOR]

目的;探讨结直肠癌（CRC）组织及结肠癌细胞DLD-1 中DEAD盒解旋酶10（DDX10）与Bystin-like（BYSL）的关系及 其临床意义。方法;收集2017 年3 月至2018 年3 月间福建医科大学附属第二医院手术切除的78 例CRC组织和配对癌旁组织， 采用免疫组织化学Envision 法检测癌组织和癌旁组织中DDX10 和BYSL 的表达水平，采用卡方检验、Pearson 相关性分析和 Kaplan-Meier 法分别分析CRC组织中DDX10、BYSL蛋白表达与其患者的临床病例特征的关系，两者表达的相关性，以及与患者 PFS和OS的关系。利用瞬时转染技术在DLD-1 细胞中分别转染siDDX10 和siNC，用qPCR法检测DLD-1 细胞中敲减DDX10 的 表达对BYSL表达的影响，用CCK-8 及Transwell实验验证DDX10 及BYSL表达对结肠癌细胞增殖迁移及侵袭的影响。结果;免 疫组织化学Envision 法检测显示，与癌旁组织相比，DDX10 和BYSL在CRC组织中呈高表达（73.08%和74.36%）。CRC组织中 DDX10 与BY肿瘤分期、是否发生淋巴结转移及复发等临床病例特征均有关联（均P<0.05）。Pearson 相关分析结果显示，DDX10 与BYSL的表达呈正相关（r=0.636，P<0.001）。Kaplan-Meier 生存分析结果显示，DDX10 与BYSL的高表达与患者更差的预后相 关。Logistic 回归分析发现，DDX10 与BYSL 均是CRC 复发的独立风险因素。CCK-8 法及Transwell 实验检测结果显示，敲降 DDX10 的表达可使BYSL表达降低，且可抑制DLD-1 细胞的增殖、迁移、侵袭能力（均P<0.05）。结论;DDX10 和BYSL在CRC 组织中呈高表达，两者表达水平呈正相关，且与患者更差的预后相关，是CRC复发的独立风险因素。可调控结直肠癌DLD-1 细 胞的增殖、迁移、侵袭能力. [ABSTRACT FROM AUTHOR]

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