Дисперсия на смесени микробни биофилми с помощта на хидролитични ензими, синтезирани от щамове Bacillus subtilis 170. (Bulgarian)

Biofilms, that are the community of microorganisms attached on biotic or abiotic surfaces coated with a self-produced matrix, composed by exopolymeric substances, dominate in all habitats on the surface of the Earth, except in the oceans, accounting for 80% of bacterial and archaeal cells. Hydrolytic enzymes have a number of industrial applications and have been indicated as an alternative to the traditional chemical methods that are used to eradicate microbial biofilms. In this study, we evaluated the ability of enzymatic extracts produced by Bacillus subtilis 170 to remove multispecies biofilms, formed by the interaction of Bacillus subtilis and Escherichia coli strains. After culture in liquid medium, containing inorganic nitrogen sources, the bacterial hydrolytic extracts showed protease (250 U/mL) activity. Cell-free supernatants of B.subtilis 170 strains with proteolytic activity were the most effective, and promoted the complete removal of Bacillus subtilis and Escherichia coli dual-species biofilms. Of the treatments using cell-free supernatants of B.subtilis 170 with proteolytic enzyme activities with 250 U/mL, total biofilm degradation was observed for both dual-species biofilms in this study. Thus, the hydrolases produced by Bacillus subtilis 170 strains evaluated here are highlighted as an interesting tool in the fight against microbial biofilms. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Mountain Agriculture on the Balkans (JMAB) is the property of Research Institute of Mountain Stockbreeding & Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)