Kaempferol inhibits airway inflammation induced by allergic asthma through NOX4-Mediated autophagy.
In: Human & Experimental Toxicology, Jg. 42 (2023), S. 1-12
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Zugriff:
Background: Kaempferol has important medicinal value in the treatment of asthma. However, its mechanism of action has not been fully understood and needs to be explored and studied. Methods: A binding activity of kaempferol with nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) was analyzed by molecular docking. Human bronchial epithelial cells (BEAS-2B) were treated with different concentrations (0, 1, 5, 10, 20, 40 μg/mL) of kaempferol to select its suitable concentration. In the transforming growth factor (TGF)-β1-induced BEAS-2B, cells were treated with 20 μg/mL kaempferol or 20 μM GLX35132 (a NOX4 inhibitor) to analyze its effects on NOX4-mediated autophagy. In the ovalbumin (OVA)-induced mice, 20 mg/kg kaempferol or 3.8 mg/kg GLX351322 administration was performed to analyze the therapeutic effects of kaempferol on NOX4-mediated autophagy. An autophagy activator, rapamycin, was used to confirm the mechanism of kaempferol in treatment of allergic asthma. Results: A good binding of kaempferol to NOX4 (score = −9.2 kcal/mol) was found. In the TGF-β1-induced BEAS-2B, the NOX4 expression was decreased with kaempferol dose increase. The secretions of IL-25 and IL-33, and the NOX4-mediated autophagy were significantly decreased by kaempferol treatment in the TGF-β1-induced BEAS-2B. In the OVA-challenged mice, kaempferol treatment improved airway inflammation and remodeling through suppressing NOX4-mediated autophagy. The rapamycin treatment clearly hampered the therapeutic effects of kaempferol in the TGF-β1-induced cells and OVA-induced mice. Conclusions: This study identifies kaempferol binds NOX4 to perform its functions in the treatment of allergic asthma, providing an effective therapeutic strategy in the further treatment of asthma. [ABSTRACT FROM AUTHOR]
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Titel: |
Kaempferol inhibits airway inflammation induced by allergic asthma through NOX4-Mediated autophagy.
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Autor/in / Beteiligte Person: | Xu, Jianfeng ; Yu, Zhenyu ; Li, Wei |
Zeitschrift: | Human & Experimental Toxicology, Jg. 42 (2023), S. 1-12 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 0960-3271 (print) |
DOI: | 10.1177/09603271231154227 |
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