Involvement of Nitric Oxide-Mediated Intrinsic Pathway Signaling in Age-Related Increase in Germ Cell Apoptosis in Male Brown-Norway Rats.
In: Journals of Gerontology Series A: Biological Sciences & Medical Sciences, Jg. 60 (2005-06-01), Heft 6, S. 702-708
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Zugriff:
We examined, using young and old Brown-Norway rats, the involvement of the nitric oxide (NO)-mediated intrinsic pathway signaling in age-related activation of male germ-cell apoptosis. Increased apoptosis of germ cells was readily observed in the normal-looking testes of old rats. Testicular NO synthase (NOS) activity, assessed by measuring the synthesis of ³H-L-citrulline from ³H-L-arginine, and cytokine-inducible NO synthase (iNOS) levels, assessed by western blot assay, were increased significantly by 90% and 70%, respectively, in the old rats compared to that of young animals. Immunohistochemical analysis of age-related changes in the expression of iNOS in testes confirmed our findings based on western blot assay. Increased NO and germ-cell apoptosis during aging is further associated with cytosolic translocation of mitochondrial cytochrome c and poly (ADP) ribose polymerase (PARP) cleavage, thus, suggesting the involvement of NO-mediated intrinsic pathway signaling in age-related increase in germ-cell apoptosis in male Brown-Norway rats. [ABSTRACT FROM AUTHOR]
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Titel: |
Involvement of Nitric Oxide-Mediated Intrinsic Pathway Signaling in Age-Related Increase in Germ Cell Apoptosis in Male Brown-Norway Rats.
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Autor/in / Beteiligte Person: | Sinha Hikim, Amiya P. ; Vera, Yanira ; Vernet, Dolores ; Castanares, Mark ; Diaz-Romero, Maruja ; Ferrini, Monica ; Swerdloff, Ronald S. ; Gonzalez-Cadavid, Nestor F. ; Wang, Christina ; Smith, James R. |
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Zeitschrift: | Journals of Gerontology Series A: Biological Sciences & Medical Sciences, Jg. 60 (2005-06-01), Heft 6, S. 702-708 |
Veröffentlichung: | 2005 |
Medientyp: | academicJournal |
ISSN: | 1079-5006 (print) |
DOI: | 10.1093/gerona/60.6.702 |
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