掌叶大黄（Rheum palmatum L.）WRKY 基因家族鉴定 与分析. (Chinese)

【Objective】This study provides a basis for the functional verification of WRKY genes, and then provide a scientific basis for the transcriptional regulation mechanism research into rhubarb quality formation.【Method】In this study, we identified WRKY gene family members in R. palmatum L. based on full-length transcriptome data, and performed a series of bioinformatics analysis, including physicochemical properties, protein domains and phylogenetic tree. RNA-seq was used for gene tissue expression analysis, along with expression profile in response to MeJA treatment.【Result】53 gene members in RpWRKYs family with complete ORFs, and the numbers of amino acids encoding the protein ranged from 192 to 748 bp, all were hydrophilic proteins, and the predicted localization was all in the nucleus. RpWRKYs combined with A. thaliana WRKYs were clustered into three groups, i.e., subgroup I, II and III, and group II with the highest number of WRKYs, accounting for 58.49%. The RNA-seq analysis revealed that RpWRKYs were differentially expressed in the roots, rhizomes and leaves of R. palmatum L., seven of them genes were highly expressed in the roots and rhizomes. Twelve RpWRKY genes showed differential expressions in the response to MeJA treatment, ten of them were significantly induced by MeJA. The expression profile of RpWRKY5, RpWRKY6, RpWRKY9 and RpWRKY33 verified by qPCR was almost consistent with the transcriptome results. RpWRKY2, RpWRKY16, RpWRKY20, RpWRKY22 and RpWRKY23 interacted with chalcone synthase (CHS) gene.【Conclusion】Expression profiles of 53 R. palmatum L. WRKY genes, bioinformatics, tissues and response to MeJA were obtained, five of which may be related to the synthesis of rhubarb anthraquinones. [ABSTRACT FROM AUTHOR]

摘 要：【目的】本研究为开展 WRKY 基因功能验证提供基础，进而为大黄药材品质形成的转录调控机制研究提供科学依据。 【方法】基于掌叶大黄（R. palmatum L.）的全长转录组数据，通过生物信息学方法鉴定掌叶大黄 WRKY 基因家族成员，分析其蛋 白理化性质、结构域、系统发育关系、蛋白互作，结合转录组数据进行不同组织和茉莉酸甲酯（methyl jasmonate, MeJA）处理的表 达谱分析。【结果】掌叶大黄 WRKY 基因家族包含 53 个成员，编码蛋白氨基酸数量为 192-748，均为亲水性蛋白，预测定位均在 细胞核。掌叶大黄 WRKY 基因家族与拟南芥 WRKY 基因家族成员进化树可分为Ⅰ、Ⅱ、Ⅲ 3 个亚族，其中Ⅱ亚族 WRKY 占比最 高（58.49%）。转录组数据分析显示掌叶大黄 WRKY 基因家族成员在掌叶大黄根、根茎和叶中差异表达，其中 7 个基因在根和根 茎中表达量较高，12 个基因响应 MeJA 处理呈差异表达，其中 10 个被 MeJA 显著诱导，4 个候选基因 RpWRKY5/6/9/33 的 qPCR 分 析与其转录组结果基本一致。蛋白互作网络显示 RpWRKY2/16/20/22/23 与查尔酮合酶（chalcone synthase, CHS）发生相互作用。【结 论】获得 53 个掌叶大黄 WRKY 基因、生物信息、组织及响应 MeJA 的表达特征，其中 5 个可能与大黄蒽醌类物质的合成有关。 [ABSTRACT FROM AUTHOR]

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