ویروس 2-SARS-CoV با اختلال در تنظیم بیان ژنهای 1-VCAM-1 ،ICAM و VWF موجب اختلال عملکرد اندوتلیال میشود. (Persian)
In: Koomesh: Journal of Semnan University of Medical Sciences, Jg. 25 (2023-09-01), Heft 5, S. 241-242
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Zugriff:
Introduction: SARS-CoV-2 was first reported in Wuhan in December 2019. It infects the target cell by binding to the ACE2. This enzyme is present in various tissues mainly in the capillaries of the lungs. Evidence suggests that symptoms of COVID-19 infection resemble the clinical phenotype of endothelial dysfunction. Endothelial dysfunction appears to be the common denominator of several clinical aspects of severe COVID-19 disease. Severe forms of Covid-19 are associated with cytokine storm syndrome (CSS). CSS stimulates adhesion molecules such as ICAM-1 and VCAM-1, which increase the adhesion of monocytes to endothelial cells and lead to endothelial activity and dysfunction. In addition, VWF is also a marker of endothelial activation, which is massively released after inflammatory vascular damage. It causes platelet aggregation, endothelial cell activation, vascular integrity defects, leukocyte proliferation, tissue inflammation, and cell damage. Methods and Materials: Five ml of tracheobronchial secretions from 34 COVID-19 patients (Alpha and Omicron, n=17) of the ICU and 40 adult patients without COVID-19 of endoscopy-department of Emam-Reza-hospital were collected. Cells are separated by centrifuge and TriPure is added to them, stored at –70 °C. Total RNA was extracted and cDNA was synthesized then primers and probes were designed for ICAM-1, VCAM-1, and VWF. Real-time PCR was performed. Data were analyzed (Ethics identifier of MUMS: IR.MUMS.MEDICAL.REC.1401.500). Results: ICAM-1 expression in the Covid-alpha group increased compared to the control (p<0.01). Expression of ICAM-1 in omicron was higher than control, but it was not significant. VCAM-1 expression in both Covid-alpha and omicron increased compared to the control (p<0.05 and p<0.001 respectively). VWF expression increased in the omicron group compared to the control (p<0.001). Conclusion: Covid-19 virus increases the expression and secretion of ICAM-1, VCAM-1, and VWF genes due to the CSS. These factors increase the adhesion of leukocytes to endotheliocytes and increase activity and dysfunction of the endothelium. [ABSTRACT FROM AUTHOR]
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ویروس 2-SARS-CoV با اختلال در تنظیم بیان ژنهای 1-VCAM-1 ،ICAM و VWF موجب اختلال عملکرد اندوتلیال میشود. (Persian)
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Autor/in / Beteiligte Person: | عرب, زهره ; فاطمه سادات محمد ; نیازمند, سعید ; سید عبدالرحیم رض ; امیر هاشم اثنی عش ; شریعتی, علیرضا |
Zeitschrift: | Koomesh: Journal of Semnan University of Medical Sciences, Jg. 25 (2023-09-01), Heft 5, S. 241-242 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 1608-7046 (print) |
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