高原 IBS 与肠屏障损伤、炎性介质及神经递质的相关性研究. (Chinese)

Objective To analyzing changes of indicators related to intestinal barrier damage, neurotransmitters and inflammatory mediators in patients with irritable bowel syndrome (IBS) in the plateau environment, and explore the underlying pathogenesis of IBS. Methods A prospective cohort observation was conducted on 81 healthy male Han Chinese volunteers who were admitted to Lhasa of Tibe from plain. The study lasted for one year. During the one-year follow-up, 13 patients who developed IBS (Rome IV) were selected as the study subjects. Additionally 11 participants who remained healthy were randomly selected as the control group (named NC). Eleven participants who entered Lhasa from plain within one week and did not develop acute mountain sickness were randomly selected as the NC early group. Serum levels of diamine oxidase (DAO) and intestinal fatty acid-binding protein (I-FABP), 5-hydroxytryptamine (5-HT), interleukin (IL)-6, IL-17A and lipopolysaccharide (LPS) were measured using enzyme-linked immunosorbent assay (ELISA). At the end of the follow-up period, colonoscopy and intestinal mucosal biopsy were performed in 9 cases of IBS, while colonoscopy was performed in 4 cases of NC. Results Colonoscopy and pathological results of IBS patients in plateau environment suggested mild inflammation of intestinal mucosa. Compared to the NC early group, the NC group showed increased levels of 5-HT, DAO, I-FABP, IL-6, IL-17A and LPS at 3 months after entering Lassa, and increased levels of 5-HT, I-FABP, IL-6, and LPS at 6 months (P＜0.05). Compared with the NC group, the IBS group showed decreased levels of 5-HT and LPS at 3 months after entering Lhasa (P＜0.05). Serological indexes of NC group at 6 months were lower than those at 3 months in the NC group (P＜0.05). There were no significant differences in serological indexes at 3 and 6 months of the IBS group. Conclusion Indicators of intestinal mucosal barrier damage, neurotransmitters and inflammatory mediators might be involved in the development of IBS in plateau environment. [ABSTRACT FROM AUTHOR]

目的 分析高原环境下肠易激综合征（IBS）患者的肠屏障损伤指标、神经递质及炎性介质变化，探讨 IBS 的潜在发病机制。方法 81 例男性汉族健康志愿者自平原进入西藏拉萨并完成 1 年前瞻性队列观察，其中诊断 IBS （罗马Ⅳ）13 例（IBS 组），并随机抽取 11 例健康者为对照组（NC 组），另随机抽取从平原进入拉萨 1 周内且未发生急性 高山病者 11 例为 NC 早期组。采用酶联免疫吸附试验（ELISA）检测血清二胺氧化酶（DAO）和肠脂肪酸结合蛋白（I-FABP）、5 羟色胺（5-HT）、白细胞介素（IL）-6、IL-17A 及脂多糖（LPS）水平；随访末月，IBS 中 9 例行肠镜检查及肠黏膜 病理活检，NC 组中 4 例行肠镜检查。结果 高原环境下 IBS 患者肠镜及病理结果提示肠黏膜轻度炎症表现。与 NC 早期组比较，NC 组进入拉萨后 3 个月时 5-HT、DAO、I-FABP、IL-6、IL-17A 和 LPS 水平及 6 个月时 5-HT、I-FABP、IL-6 和 LPS 水平均升高，IBS 组 3、6 个月时 6 项血清学指标均升高（P＜0.05）；与 NC 组比较，IBS 组 3 个月时 5-HT 和 LPS 水平降低，6 个月时 DAO、I-FABP、IL-6、IL-17A 和 LPS 水平升高（P＜0.05）。NC 组 6 个月时 6 项血清学指标均较 3 个 月时降低（P＜0.05）；IBS 组 3 个月与 6 个月时血清学指标差异均无统计学意义。结论 肠黏膜屏障损伤指标、神经递 质及炎性介质可能参与高原环境下 IBS 的发生。 [ABSTRACT FROM AUTHOR]

Copyright of Tianjin Medical Journal is the property of Tianjin Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)