Patent Issued for Nucleic acid loaded extracellular vesicles (USPTO 11970718).

Carmine Therapeutics Pte. Ltd. has been issued a patent for a method of loading nucleic acid cargo, such as DNA, into extracellular vesicles (EVs) for use in therapy and research. EVs are cell-derived vesicles that can transport biomolecules between cells and are being explored as drug delivery vehicles. This patent provides details on the types of nucleic acids that can be loaded, their lengths, and the methods used for loading. The patent specifically describes a preparation of red blood cell extracellular vesicles (RBCEVs) loaded with DNA cargo and a cationic reagent. The RBCEVs have a size between 50 nm and 1,000 nm in diameter, and the DNA cargo can have a length between 2,000 bases and 40,000 bases. The preparation is more effective at delivering the DNA cargo to target cells compared to a reference preparation loaded by electroporation. The patent also includes methods for loading RBCEVs with DNA cargo using a polyethylenimine (PEI) reagent. [Extracted from the article]

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