Gene expression profile, pathways, and transcriptional system regulation in indolent systemic mastocytosis.
In: Allergy, Jg. 66 (2011-02-01), Heft 2, S. 229-237
Online
academicJournal
Zugriff:
Background: Mastocytosis is an uncommon disease resulting from proliferation of abnormal mast cells infiltrating skin, bone marrow, liver, and other tissues. The aim of this study was to find differences in gene expression in peripheral blood cells of patients with indolent systemic mastocytosis compared to healthy controls. The second aim was to define a specific gene expression profile in patients with mastocytosis. Methods: Twenty-two patients with indolent systemic mastocytosis and 43 healthy controls were studied. Whole genome gene expression analysis was performed on RNA samples isolated from the peripheral blood. For amplification and labelling of the RNA, the Illumina TotalPrep 96 RNA Amplification Kit was used. Human HT-12_V3_expression arrays were processed. Data analysis was performed using GeneSpring, Genecodis, and Transcriptional System Regulators. Results: Comparison of gene expression between patients and controls revealed a significant difference (P < 0.05 corrected for multiple testing) and the fold change difference >2 in gene expression in 2303 of the 48.794 analysed transcripts. Functional annotation indicated that the main pathways in which the differently expressed genes were involved are ubiquitin-mediated proteolysis, MAPK signalling pathway, pathways in cancer, and Jak-STAT signalling. The expression distributions for both groups did not overlap at all, indicating that many genes are highly differentially expressed in both groups. Conclusion: We were able to find abnormalities in gene expression in peripheral blood cells of patients with indolent systemic mastocytosis and to construct a gene expression profile which may be useful in clinical practice to predict the presence of mastocytosis and in further research of novel drugs. [ABSTRACT FROM AUTHOR]
Copyright of Allergy is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Gene expression profile, pathways, and transcriptional system regulation in indolent systemic mastocytosis.
|
---|---|
Autor/in / Beteiligte Person: | Niedoszytko, Marek ; Oude Elberink, J. N. G. ; Bruinenberg, M. ; Nedoszytko, B. ; de Monchy, J. G. R. ; te Meerman, G. J. ; Weersma, R. K. ; Mulder, A. B. ; Jassem, E. ; van Doormaal, J. J. |
Link: | |
Zeitschrift: | Allergy, Jg. 66 (2011-02-01), Heft 2, S. 229-237 |
Veröffentlichung: | 2011 |
Medientyp: | academicJournal |
ISSN: | 0105-4538 (print) |
DOI: | 10.1111/j.1398-9995.2010.02477.x |
Schlagwort: |
|
Sonstiges: |
|