Effects of Ginkgolide A on Okadaic Acid-induced Tau Hyperphosphorylation and the PI3K-Akt Signaling Pathway in N2a Cells.
In: Planta Medica, Jg. 78 (2012-08-15), Heft 12, S. 1337-1341
serialPeriodical
Zugriff:
Alzheimer's disease is the most common form of dementia leading to the irreversible loss of neurons, and Tau hyperphosphorylation has an important role in the pathology of Alzheimer's disease. Ginkgolide A is one of the active components of Ginkgo biloba extracts which has been proven to have neuroprotective effects, but the effect of ginkgolide A on Tau hyperphosphorylation has not yet been reported. In this study, the effects of ginkgolide A on cell viability, Tau hyper-phosphorylation, and the PI3K-Akt signaling pathway in N2a cell lines were explored, and methods such as the MTT assay, ELISA, and Western blots techniques were used. The results showed that ginkgolide A could increase cell viability and suppress the phosphorylation level of Tau in cell lysates, meanwhile, GSK3β was inhibited with phosphorylation at Ser9. Moreover, treatment of the cellswith ginkgolide A promoted phosphorylation of PI3K and Akt, suggesting that the activation of the PI3K-Akt signaling pathway may be the mechanism for ginkgolide A to prevent the intracellular accumulation of p-Tau induced by okadaic acid and to protect the cells from Tau hyperphosphorylation-related toxicity. [ABSTRACT FROM AUTHOR]
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Titel: |
Effects of Ginkgolide A on Okadaic Acid-induced Tau Hyperphosphorylation and the PI3K-Akt Signaling Pathway in N2a Cells.
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Autor/in / Beteiligte Person: | Chen, Yan ; Wang, Cui ; Hu, Meili ; Pan, Jian ; Chen, Jianhua ; Duan, Peilu ; Zhai, Tianlong ; Ding, Jingna ; Xu, Cunji |
Zeitschrift: | Planta Medica, Jg. 78 (2012-08-15), Heft 12, S. 1337-1341 |
Veröffentlichung: | 2012 |
Medientyp: | serialPeriodical |
ISSN: | 0032-0943 (print) |
DOI: | 10.1055/s-0032-1314965 |
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