Th17 Immunity in Children with Allergic Asthma and Rhinitis: A Pharmacological Approach.
In: PLoS ONE, Jg. 8 (2013-04-01), Heft 4, S. 1-13
Online
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Zugriff:
Th17 cells and IL-17A play a role in the development and progression of allergic diseases. We analyzed the IL-17A levels in sputum supernatants (Ss), nasal wash (NW) and plasma (P) from Healthy Controls (HC) and children with Asthma/Rhinitis. We tested the expression of IL-17A, RORγ(t) and FOXP3 in peripheral blood T-lymphocytes from intermittent and mild-moderate asthma. The effect of Budesonide and Formoterol was tested “in vitro” on IL-17A, RORγ(t) and FOXP3 expression in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis patients, and on nasal and bronchial epithelial cells stimulated with NW and Ss from mild-moderate asthma/persistent rhinitis. Further, the effect of 12 weeks of treatment with Budesonide and Formoterol was tested “in vivo” in T-lymphocytes from mild-moderate asthma/persistent rhinitis patients. IL-17A was increased in Ss, NW and P from children with mild-moderate asthma compared with intermittent and HC. In cultured T-lymphocytes IL-17A and RORγ(t) expression were higher in mild-moderate asthma/persistent rhinitis than in mild-moderate asthma/intermittent rhinitis, while FOXP3 was reduced. Budesonide with Formoterol reduced IL-17A and RORγ(t), while increased FOXP3 in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis, and reduced the IL-8 release mediated by IL-17A present in NW and Ss from mild-moderate asthma/persistent rhinitis in nasal and bronchial epithelial cells. Finally, Budesonide with Formoterol reduced IL-17A levels in P and Ss, CD4 + IL-17A + T-cells, in naïve children with mild-moderate asthma/persistent rhinitis after 12 weeks of treatment. Th17 mediated immunity may be involved in the airway disease of children with allergic asthma and allergic rhinitis. Budesonide with Formoterol might be a useful tool for its therapeutic control. [ABSTRACT FROM AUTHOR]
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Titel: |
Th17 Immunity in Children with Allergic Asthma and Rhinitis: A Pharmacological Approach.
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Autor/in / Beteiligte Person: | Albano, Giusy Daniela ; Di Sano, Caterina ; Bonanno, Anna ; Riccobono, Loredana ; Gagliardo, Rosalia ; Chanez, Pascal ; Gjomarkaj, Mark ; Montalbano, Angela Marina ; Anzalone, Giulia ; La Grutta, Stefania ; Ricciardolo, Fabio Luigi Massimo ; Profita, Mirella |
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Zeitschrift: | PLoS ONE, Jg. 8 (2013-04-01), Heft 4, S. 1-13 |
Veröffentlichung: | 2013 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (print) |
DOI: | 10.1371/journal.pone.0058892 |
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