The Study of P53 Polymorphism in Codon 72 in Patients with Thyroid Cancer in a Report from East Azerbaijan Province IRAN. (English)

Backgrounds and Objectives: Thyroid cancer is the most common malignancy of the endocrine system. It seem that Ethnic and geographic factors are important elements. Cancer associated with mutation in tumor suppressor genes and oncogene. In addition to mutation, genetic polymorphisms are involved in incidence of cancer. The purpose of this investigation was studying polymorphism at codon 72 of p53 gene in patients with thyroid cancer in East Azerbaijan province. Material and Methods: In a case-control study 60 patients with thyroid cancer and 99 healthy individuals were studied as controls. After DNAs extraction from peripheral blood cells different genotypes of the 72th codon of p53 gene were determined by amplification refractory mutation system (ARMS) and presence of probable mutation, single strand conformation polymorphism (SSCP) technique was also conducted to confirm the results; Exon 4 of p53 gene was investigated by DNA sequencing. Results: In the control group, the genotype distribution of p53 polymorphism for Arg/Arg, Arg/Pro and Pro/Pro genotypes was 30.3%, 50.5% and 19.2% respectively. In the caner groups, the distribution was 31.67%, 51.67%, 16.67%, respectively for the same order of genotypes. Conclusion: This study indicates that polymorphism at codon 72 p53 gene is not a genetic predisposing factor for thyroid cancer in this population. [ABSTRACT FROM AUTHOR]

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