PROLIFERAÇÃO CELULAR, APOPTOSE, EXPRESSÃO DA CICLINA A E DO P21 NO TUMOR DE EHRLICH SOB EFEITO DO HIPOTIREOIDISMO ASSOCIADO OU NÃO À CASTRAÇÃO. (Portuguese)

Seeking to study the possible mechanism of action of thyroid hormones and the inter-relationship between thyroid-gonad on cancer, we investigated the effect of hypothyroidism and castration in Ehrlich tumor. We analyzed the rates of cell proliferation, apoptosis and expression of cyclin A and p21. We used 32 tumors inoculated in the footpad of female mice divided into euthyroid and hypothyroid castrated or not. Histological sections of the tumor were subjected to immunohistochemistry with markers of cell proliferation and cycle regulatory proteins, and HE staining to determine apoptosis. A significant expression of p21 and proliferation in hypothyroid animals. It was concluded that increased expression of p21 in hypothyroid probably promoted extension or cell cycle arrest. [ABSTRACT FROM AUTHOR]

Buscando estudar o possível mecanismo de ação dos hormônios tireoidianos e a inter-relação tireóide-gônadas sobre o câncer, investigou-se o efeito do hipotireoidismo e da castração no tumor de Ehrlich. Foram analisados os índices de proliferação celular, apoptose e expressões da ciclina A e da p21. Utilizaram-se 32 tumores inoculados no coxim plantar de camundongos fêmeas divididos em: hipotireóideo e eutireóideos castrados ou não. Secções histológicas do tumor foram submetidas à imunoistoquímica com marcadores de proliferação celular e proteínas reguladoras do ciclo, e coloração de HE para determinação da apoptose. Observouse significativa expressão da p21 e da proliferação nos animais hipotireóideos. Conclui-se que a maior expressão da p21 nos hipotireóideos, provavelmente, promoveu o prolongamento ou bloqueio do ciclo celular. [ABSTRACT FROM AUTHOR]

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