Interleukin 17 Induces Up-Regulation of Chemokine and Cytokine Expression Via Activation of the Nuclear Factor κB and Extracellular Signal-Regulated Kinase 1/2 Pathways in Gynecologic Cancer Cell Lines.
In: International Journal of Gynecological Cancer, Jg. 21 (2011-12-01), Heft 9, S. 1533-1539
academicJournal
Zugriff:
Previous studies have revealed that interleukin 17 (IL-17) contributes to pathological processes in many solid tumors. However, the roles of IL-17 in gynecologic cancer still remain elusive, hindering the deep understanding of gynecologic tumorigenesis.In the present study, to delineate the functional roles of IL-17 in gynecologic cancer, IL-17 stimulation was introduced in cell lines of 3 gynecologic cancers, and IL-17-induced expression of chemokines and cytokines and possible signaling pathways were investigated.Our results showed that in HEC-1-B (human endometrial cancer) cells, IL-17 stimulation induced mRNA level increases of CCL2, CCL5, CCL20, CXCL2, and IL-8. Similar treatment in HeLa cells caused increases in the mRNA levels of CCL2, CXCL2, IL-6, and IL-8, and in SKOV3 cells, mRNA levels of CCL2, CCL20, CXCL1, CXCL2, IL-6, and IL-8 increased. The increases in mRNA levels induced by IL-17 were dose- and time-dependent. Furthermore, with the addition of the NF-κB (nuclear factor κ-light-chain-enhancer of activated B) and extracellular signal-regulated kinase inhibitors pyrrolidine dithiocarbamate and PD98059, the IL-17-induced CCL2 mRNA level was significantly compromised. IL-17 stimulation also activated phosphorylation of IκBα and extracellular signal-regulated kinase 1/2 in a time-dependent manner.These results demonstrated that IL-17 may regulate chemokines and cytokines in gynecologic cancers. [ABSTRACT FROM AUTHOR]
Copyright of International Journal of Gynecological Cancer is the property of BMJ Publishing Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Interleukin 17 Induces Up-Regulation of Chemokine and Cytokine Expression Via Activation of the Nuclear Factor κB and Extracellular Signal-Regulated Kinase 1/2 Pathways in Gynecologic Cancer Cell Lines.
|
---|---|
Autor/in / Beteiligte Person: | Lai, Ting ; Wang, Kana ; Hou, Qiannan ; Zhang, Jian ; Yuan, Jialing ; Yuan, Lixing ; You, Zongbing ; Xi, Mingrong |
Zeitschrift: | International Journal of Gynecological Cancer, Jg. 21 (2011-12-01), Heft 9, S. 1533-1539 |
Veröffentlichung: | 2011 |
Medientyp: | academicJournal |
ISSN: | 1048-891X (print) |
DOI: | 10.1097/IGC.0b013e31822d2abd |
Sonstiges: |
|