Transformation of 17β-estradiol in humic acid solution by ε-MnO2 nanorods as probed by high-resolution mass spectrometry combined with 13C labeling.
In: Environmental Pollution, Jg. 214 (2016-07-01), S. 211-218
academicJournal
Zugriff:
Steroidal estrogens (SEs), widespread in aquatic systems, have a potential to disrupt the endocrine system of wildlife species and humans. In our experiments, the performance of ε -MnO 2 nanorods in transforming 17 β -estradiol (E2) was investigated, and the effect of humic acid (HA) on the reaction behaviors was systematically characterized. Reconfiguration of humic molecules was also investigated by high-performance size exclusion chromatography (HPSEC). Results indicated that ε -MnO 2 nanomaterials ensured efficient removal of E2 from the aqueous solution. The presence of HA hindered the transformation of E2, while enhanced the cross-coupling between E2 and humic molecules. In particular, we used a mixture of un-labeled E2 and 13 C 3 -labeled E2 at a 1: 1 set ratio (w/w) to probe the reaction products via high-resolution mass spectrometry (HRMS). The combination of HRMS and 13 C 3 -labeling revealed the intermediate products including estrone (E1), and hydroxylated, quinone-like, and ring-opened species, as well as E2 dimer and trimer. More importantly, possible cross-coupling products between E2 and HA were also identified. A reaction mechanism including two-electron oxidation and single-electron oxidation was proposed. The applied analytical approach using HRMS along with 13 C 3 -labeling for reaction-product identification is crucial to understanding the role of HA in the transformation of SEs. [ABSTRACT FROM AUTHOR]
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Transformation of 17β-estradiol in humic acid solution by ε-MnO2 nanorods as probed by high-resolution mass spectrometry combined with 13C labeling.
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Autor/in / Beteiligte Person: | Sun, Kai ; Liang, Shangtao ; Kang, Fuxing ; Gao, Yanzheng ; Huang, Qingguo |
Zeitschrift: | Environmental Pollution, Jg. 214 (2016-07-01), S. 211-218 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 0269-7491 (print) |
DOI: | 10.1016/j.envpol.2016.04.021 |
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