Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis.
In: Asian Pacific Journal of Tropical Biomedicine, Jg. 13 (2023-08-01), Heft 8, S. 348-358
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Zugriff:
Objective: To evaluate the effect of hydroxysafflor yellow A (HSYA) on thioacetamide-induced liver fibrosis. Methods: Thioacetamide was administered to rats intraperitoneally in doses of 200 mg/kg twice a week for 12 weeks. Thioacetamide-intoxicated rats were given silymarin (50 mg/kg) or HSYA (5 mg/kg) orally every day for 8 weeks. Liver enzymes, fibrosis markers, histological changes as well as immunohistochemistry of TNF-α, IL-6, p21, α-SMA, and caspase-3 were examined. The effect of HSYA on HSC-T6 activation/proliferation and apoptosis was also determined in vitro. Results: HSYA decreased liver enzymes, TNF-α, IL-6, and p21 expressions, hepatic PDGF-B, TIMP-1, TGF-β1, and hydroxyproline levels, as well as fibrosis score (S2 vs. S4) compared to the thioacetamide group. HSYA also downregulated α-SMA while increasing caspase-3 expression. Surprisingly, at 500 µg/mL, HSYA had only a slightly suppressive effect on HSC proliferation, with a 9.5% reduction. However, it significantly reduced TGF-β1, inhibited α-SMA expression, induced caspase-3 expression, and promoted cell senescence. Conclusions: HSYA may be a potential therapeutic agent for delaying and reversing the progression of liver fibrosis. More research on HSYA at higher doses and for a longer period is warranted. [ABSTRACT FROM AUTHOR]
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Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis.
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Autor/in / Beteiligte Person: | Seif el-Di, Sayed H. ; Hammam, Olfat A. ; Ezzat, Shahira M. ; Saleh, Samira ; Safar, Marwa M. ; El Maadawy, Walaa H. ; El-Lakkany, Naglaa M. |
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Zeitschrift: | Asian Pacific Journal of Tropical Biomedicine, Jg. 13 (2023-08-01), Heft 8, S. 348-358 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 2221-1691 (print) |
DOI: | 10.4103/2221-1691.383689 |
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