Effects of 17β-estradiol, progesterone, synthetic progestins, tibolone, and raloxifene on vascular endothelial growth factor and Thrombospondin-1 messengerRNA in breast cancer cells.
In: International Journal of Gynecological Cancer, Jg. 16 (2006-09-02), S. 560-563
academicJournal
Zugriff:
To determine the effect of 17β-estradiol, raloxifene, progesterone, medroxyprogesterone acetate (MPA), levonorgestrel (LNG), norethindrone (NET), tibolone and tibolone metabolites on vascular endothelial growth factor (VEGF) isoforms 121 and 165 and Thrombospondin-1 (TSp-1) messengerRNA (mRNA) in two breast cancer cell lines, MCF-7 and T47-D. MCF-7 and T47-D cells were cultured to 80% confluence, in vitro. After 24 h incubation in serum-free media, 1.0, 0.1, and 0.01 μM of 17β-estradiol, raloxifene, raloxifene plus ICI 182780, tibolone, 3α-hydroxytibolone, and 3β-hydroxytibolone were added to MCF-7 cells. Progesterone, MPA, LNG, NET, and Δ 4 tibolone at 1.0, 0.1, and 0.01 μM were added to T47-D cells. The cells plus steroids were incubated for a further 24 h. Total RNA was isolated using TRIZOL ® and reverse transcriptase–polymerase chain reaction was carried out using primers for VEGF, TSP-1, and cyclophilin, the latter as an internal control. Semiquantitative analysis was performed using 33 P-CTP for radioactive labeling during the polymerase chain reaction. 17β-estradiol, raloxifene, tibolone, 3α-hydroxytibolone, and 3β-hydroxytibolone had no effect on VEGF mRNA in MCF-7 cells. Progesterone, MPA, LNG, and NET increased VEGF mRNA in T47-D cells. Δ 4 tibolone also increased VEGF mRNA but to a lesser extent than the progestogens. Raloxifene increased TSP-1 mRNA, this effect was not reversed by the addition of ICI 182780 to the media. 17β-estradiol, raloxifene, tibolone and tibolone hydroxy-metabolites had no effect on VEGF mRNA in MCF-7 cells. Progesterone and progestins increased VEGF mRNA in T47-D breast cancer cells. Δ 4 tibolone was less effective than progestogens on this angiogenic gene in the T47-D cells. Raloxifene increased TSP-1. These differential effects may be related to breast cancer growth. [ABSTRACT FROM AUTHOR]
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Titel: |
Effects of 17β-estradiol, progesterone, synthetic progestins, tibolone, and raloxifene on vascular endothelial growth factor and Thrombospondin-1 messengerRNA in breast cancer cells.
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Autor/in / Beteiligte Person: | Mirkin, S. ; Wong, B. C. ; Archer, D. F. |
Zeitschrift: | International Journal of Gynecological Cancer, Jg. 16 (2006-09-02), S. 560-563 |
Veröffentlichung: | 2006 |
Medientyp: | academicJournal |
ISSN: | 1048-891X (print) |
DOI: | 10.1111/j.1525-1438.2006.00696.x |
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